Gadelhaq Sahar M, Arafa Waleed M, Abolhadid Shawky M
Department of Parasitology, Faculty of Veterinary Medicine, Minia University, El-Minia, Egypt.
Department of Parasitology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, 62511, Egypt.
Vet Parasitol. 2018 Feb 15;251:12-16. doi: 10.1016/j.vetpar.2017.12.020. Epub 2017 Dec 26.
This study was designed to investigate the ability of two herbal extracts and different chemical substances to inhibit or disrupt sporulation of Eimeria species oocysts of the chickens. The two herbal extracts were Allium sativum (garlic) and Moringa olifiera while the chemical substances included commercial disinfectants and diclazuril. Field isolates of Eimeria oocysts were propagated in chickens to obtain a continuous source of oocysts. The collected unsporulated oocysts (10oocysts/5 ml) were dispensed into 5 cm Petri dish. Three replicates were used for each treatment. The treated oocysts were incubated for 48 h at 25-29 °C and 80% relative humidity. The results showed that herbal extracts, the commercial recommended dose of Dettol, TH4, Phenol, VirkonS, and Diclazuril 20% have no effect on the sporulation. While Sodium hypochlorite showed a significant degree of sporulation inhibition reached to 49.67%. Moreover, 70% ethanol, and 10% formalin showed 100% sporulation inhibition. It was concluded that 70% ethanol and 10% formalin are the most effective methods to inhibit Eimeria species sporulation.
本研究旨在调查两种草药提取物和不同化学物质抑制或破坏鸡艾美球虫种卵囊孢子化的能力。两种草药提取物为大蒜和辣木，化学物质包括商业消毒剂和地克珠利。艾美球虫卵囊的田间分离株在鸡体内繁殖以获得连续的卵囊来源。将收集到的未孢子化卵囊（10个卵囊/5毫升）放入5厘米的培养皿中。每种处理设置三个重复。处理后的卵囊在25-29°C和80%相对湿度下孵育48小时。结果表明，草药提取物、滴露商业推荐剂量、TH4、苯酚、卫可、20%地克珠利对孢子化没有影响。而次氯酸钠显示出显著的孢子化抑制程度，达到49.67%。此外，70%乙醇和10%福尔马林显示出100%的孢子化抑制。得出的结论是，70%乙醇和10%福尔马林是抑制艾美球虫种孢子化的最有效方法。
