Tchodo Ferdinand Grégoire, Dakpogan Hervé Brice, Sanvee Sabrina, Adjei-Mensah Benjamin, Kpomasse Claude Cocou, Karou Simplice, Pitala Wéré, Tona Kokou, Bakoma Batomayena
Regional Center of Excellence in Poultry Science, University of Lome, Lome BSP 1515, Togo.
School of Animal Production System Management, National Agricultural University of Ketou, Ketou P.O. Box 43, Benin.
Vet Sci. 2024 Jul 31;11(8):345. doi: 10.3390/vetsci11080345.
Coccidiosis is a parasitic disease that often affects livestock. Identifying plants with inhibitory effects on the development of the parasite could help in finding new natural treatments. This study aimed to evaluate the anticoccidial potentials of extracts from leaves (AILs), leaves (CMLs), seeds (CPSs), roots (SLRs), and leaves (VALs). The in vitro anticoccidial efficacy of the extracts was evaluated through oocyst sporulation inhibition and sporozoite viability inhibition assays of oocysts. The setup was examined for 72 h (every 24 h) of incubation. The DPPH radical scavenging activity and ferric reducing antioxidant power were used to evaluate the antioxidant potential of the extracts. Among the tested extracts, the SLR, CPS, and AIL extracts exhibited the maximum oocyst sporulation inhibition (75.85 ± 1.21%, 74.53 ± 1.65%, and 71.58 ± 0.24%, respectively) at a concentration of 75 mg/mL of plant extracts against the species. The root extract showed the highest radical scavenging capacity (76.25 ± 0.53) and reducing power (86.21 ± 4.28). The biochemical screening of the selected plant extracts revealed the presence of antioxidant compounds such as phenols, flavonoids, alkaloids, saponins, and carbohydrates. The SLR extract contained the highest amounts of phenols (56.11 ± 0.33 µg/mL) and flavonoids (36.65 ± 1.85 µg/mL). In conclusion, the selected hydro-ethanolic extracts from these plants possess excellent anticoccidial and antioxidant activities, which can be attributed to the presence of medicinally important phytochemicals. Further research is needed to identify and isolate the active anticoccidial compounds from these plants, which could be utilized in the development of drugs against coccidiosis.
球虫病是一种常影响家畜的寄生虫病。鉴定对该寄生虫发育具有抑制作用的植物有助于找到新的天然治疗方法。本研究旨在评估来自[植物名称1]叶（AILs）、[植物名称2]叶（CMLs）、[植物名称3]种子（CPSs）、[植物名称4]根（SLRs）和[植物名称5]叶（VALs）的提取物的抗球虫潜力。通过对[寄生虫名称]卵囊的卵囊孢子化抑制和子孢子活力抑制试验来评估提取物的体外抗球虫功效。在孵育72小时（每24小时）后检查试验设置。采用DPPH自由基清除活性和铁还原抗氧化能力来评估提取物的抗氧化潜力。在所测试的提取物中，SLR、CPS和AIL提取物在植物提取物浓度为75mg/mL时，对[寄生虫名称]表现出最大的卵囊孢子化抑制作用（分别为75.85±1.21%、74.53±1.65%和71.58±0.24%）。[植物名称4]根提取物表现出最高的自由基清除能力（76.25±0.53）和还原能力（86.21±4.28）。对所选植物提取物的生化筛选揭示了酚类、黄酮类、生物碱、皂苷和碳水化合物等抗氧化化合物的存在。SLR提取物含有最高含量的酚类（56.11±0.33μg/mL）和黄酮类（36.65±1.85μg/mL）。总之，从这些植物中选择的水乙醇提取物具有优异的抗球虫和抗氧化活性，这可归因于具有药用重要性的植物化学物质的存在。需要进一步研究从这些植物中鉴定和分离活性抗球虫化合物，这些化合物可用于开发抗球虫病药物。
