Department of Animal Science, North Carolina Agricultural and Technical State University, Greensboro, NC, 27411, USA.
Department of Natural Resources & Environmental Design, North Carolina Agricultural and Technical State University, Greensboro, NC, 27411, USA.
Folia Microbiol (Praha). 2021 Feb;66(1):41-48. doi: 10.1007/s12223-020-00817-1. Epub 2020 Aug 30.
A batch culture technique was used to evaluate dry matter (DM) digestibility of corn stover (Zea mays L.) and kudzu, Pueraria montana (Lour.) Merr. after solid-state fermentation (SSF) with a white-rot fungus, Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm. Five dietary treatments consisting of mixtures of corn stover (C) and kudzu (K) in varying ratios, (1) 100C:0K, (2) 75C:25K, (3) 50C:50K, (4) 25C:75K, and (5) 0C:100K, were inoculated with P. ostreatus (MBFBL 400) and subjected to SSF for 0, 35, and 77 days. The study was arranged as a 5 × 3 factorial design with 3 replicates. Grass hay was included in the study as a control. Interactions (P < 0.05) between treatments and fermentation time were noted for the fermentation kinetics. Asymptotic gas was the highest (P < 0.05) for 0C:100K and 100C:0K on day 77. Treatment effect (P < 0.001) and treatment × fermentation time interaction (P < 0.001) were noted for in vitro dry matter digestibility (IVDMD). On day 77, treatment 4 had the highest (P < 0.001) IVDMD value, while treatment 1 had the lowest (P < 0.001) IVDMD. There was no difference (P > 0.05) between treatments 3, 5, and control. Numerically, the ranking of their IVDMD values from the highest to the lowest is 4 > 2 > 5 > control >3 > 1. The results show that the treated corn stover and kudzu mixes were comparable with the control, which is good quality hay. This is the first report that demonstrates the potential use of a combined mixture of corn stover and kudzu in ruminant animal feed development.
采用批次培养技术，用白腐真菌糙皮侧耳（Pleurotus ostreatus）（Jacq. ex Fr.）P. Kumm. 对固态发酵（SSF）前后的玉米秸秆（Zea mays L.）和葛（Pueraria montana）（Lour.）Merr. 进行干物质（DM）消化率评估。5 种日粮处理组由不同比例的玉米秸秆（C）和葛（K）混合物组成，（1）100C：0K，（2）75C：25K，（3）50C：50K，（4）25C：75K，和（5）0C：100K，用糙皮侧耳（MBFBL 400）接种并进行 SSF0、35 和 77 天。该研究采用 5×3 因子设计，有 3 个重复。本研究还包括干草作为对照。注意到处理和发酵时间之间存在相互作用（P<0.05），这影响了发酵动力学。77 天时，0C：100K 和 100C：0K 的渐近气体最高（P<0.05）。处理效果（P<0.001）和处理×发酵时间相互作用（P<0.001）对体外干物质消化率（IVDMD）有影响。77 天时，处理 4 的 IVDMD 值最高（P<0.001），而处理 1 的 IVDMD 值最低（P<0.001）。处理 3、5 和对照组之间没有差异（P>0.05）。数值上，它们的 IVDMD 值从高到低的排序是 4>2>5>对照>3>1。结果表明，处理后的玉米秸秆和葛混合物与对照（优质干草）相当。这是第一个表明玉米秸秆和葛混合物在反刍动物饲料开发中具有潜在用途的报告。
