University of Fallujah College of Veterinary Medicine, Al-Anbar, Iraq.
Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetic Research, Mustansiriyah University, Baghdad, Iraq.
Intervirology. 2017;60(4):156-164. doi: 10.1159/000486594. Epub 2018 Feb 9.
This study aims to provide a molecular and epidemiological characterization of bovine papillomavirus (BPV) infections in Iraq.
The present study focuses on identifying BPV based on clinical and epidemiological manifestations, histopathological examinations, and polymerase chain reactions (PCR). Samples were collected from 163 animals suffering from cutaneous bovine papillomatosis, including 129 females (79.14%) with an age range of 16-40 months and 34 males (20.85%) with an age range of 17-29 months.
The incidence rate was significantly higher in females than in males. The most commonly affected sites were the teats and neck, though warts were found in other areas of the body. Histological sections were diagnosed as fibropapilloma. PCR results showed that 80.13% of the extracted papilloma DNA samples corresponded to the BPV-1 genotype. Furthermore, 7.94% of the samples showed a mixed infection of BPV-1 and BPV-13. While, 40.63% of the extracted DNA blood samples showed 2 DNA fragments corresponding to both genotypes BPV-1 and BPV-2.
This study confirmed the presence of BPV-1, BPV-2, and BPV-13, which belong to the Deltapapillomavirus genera, for the first time in the DNA of Iraqi cattle. Understanding BPV diversity and epidemiology is of critical importance for starting prevention strategies.
本研究旨在对伊拉克的牛乳头瘤病毒（BPV）感染进行分子和流行病学特征分析。
本研究重点基于临床和流行病学表现、组织病理学检查和聚合酶链反应（PCR）来识别 BPV。从 163 头患有皮肤牛乳头瘤病的动物中采集样本，包括 129 头雌性（79.14%），年龄在 16-40 个月之间，34 头雄性（20.85%），年龄在 17-29 个月之间。
雌性的发病率明显高于雄性。最常受影响的部位是乳头和颈部，但身体其他部位也发现了疣。组织学切片诊断为纤维乳头瘤。PCR 结果显示，80.13%的提取乳头瘤 DNA 样本与 BPV-1 基因型相对应。此外，7.94%的样本显示 BPV-1 和 BPV-13 的混合感染。而 40.63%的提取 DNA 血液样本显示出两种基因型 BPV-1 和 BPV-2 的 2 个 DNA 片段。
本研究首次在伊拉克牛的 DNA 中证实了 BPV-1、BPV-2 和 BPV-13 的存在，它们属于 Delta 乳头瘤病毒属。了解 BPV 的多样性和流行病学特征对于启动预防策略至关重要。
