Scientific and Medical Affairs, Cbr Systems, Inc., South San Francisco, California, USA.
Scientific and Medical Affairs, Cbr Systems, Inc., South San Francisco, California, USA.
Cytotherapy. 2018 Apr;20(4):564-575. doi: 10.1016/j.jcyt.2018.01.001. Epub 2018 Feb 21.
Umbilical cord (UC) tissue can be collected in a noninvasive procedure and is enriched in progenitor cells with potential therapeutic value. Mesenchymal stromal cells (MSCs) can be reliably harvested from fresh or cryopreserved UC tissue by explant outgrowth with no apparent impact on functionality. A number of stem cell banks offer cryopreservation of UC tissue, alongside cord blood, for future cell-based applications. In this setting, measuring and monitoring UC quality is critical.
UC explants were evaluated using a plating and scoring system accounting for cell attachment and proliferation. Explant scores for fresh and cryopreserved-then-thawed tissue from the same UC were compared. Metabolic activity of composite UC tissue was also assayed after exposure of the tissue to conditions anticipated to affect UC quality and compared with explant scores within the same UC.
All fresh and cryopreserved tissues yielded MSC-like cells, and cryopreservation of the tissue did not prevent the ability to isolate MSCs by the explant method. Thawed UC tissue scores were 91% (±0.6%; P = 0.0009) that of the fresh, biologically identical tissue. Within the same UC, explant scores correlated well to both cell yield (R = 0.85) and tissue metabolic activity (R = 0.69).
A uniform explant scoring assay can provide information about the quality of composite UC tissue. Such quantitative measurement is useful for analysis of tissue variability and process monitoring. Additionally, a metabolic assay of UC tissue health provides results that correlate well to explant scoring results.
脐带（UC）组织可以通过非侵入性程序收集，并且富含具有潜在治疗价值的祖细胞。通过组织块培养法，可以从新鲜或冷冻保存的 UC 组织中可靠地收获间充质基质细胞（MSCs），而对其功能没有明显影响。许多干细胞库提供冷冻保存 UC 组织，以及脐带血，以备将来进行基于细胞的应用。在这种情况下，测量和监测 UC 的质量至关重要。
使用一种平板和评分系统评估 UC 外植体，该系统考虑了细胞附着和增殖。比较了同一 UC 的新鲜和冷冻-解冻组织的外植体评分。还对组织暴露于预期影响 UC 质量的条件后的复合 UC 组织的代谢活性进行了测定，并与同一 UC 内的外植体评分进行了比较。
所有新鲜和冷冻保存的组织都产生了 MSC 样细胞，并且组织的冷冻保存并没有阻止通过外植体方法分离 MSC 的能力。解冻的 UC 组织评分与新鲜的、生物学上相同的组织评分（91%±0.6%；P=0.0009）相同。在同一 UC 内，外植体评分与细胞产量（R=0.85）和组织代谢活性（R=0.69）高度相关。
统一的外植体评分测定法可以提供有关复合 UC 组织质量的信息。这种定量测量对于组织变异性分析和过程监测非常有用。此外，UC 组织健康的代谢测定法提供的结果与外植体评分结果相关性良好。
