Tang Yuhan, Zhao Daqiu, Tao Jun
Key Laboratory of Crop Genetics and Physiology of Jiangsu Province, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou, 225009 People's Republic of China.
3 Biotech. 2018 Feb;8(2):102. doi: 10.1007/s13205-018-1127-0. Epub 2018 Jan 29.
Daminozide is a plant growth retardant that inhibits 2-oxoglutarate-dependent dioxygenase activity in the gibberellin biosynthesis pathway, but few studies have focused on its effect on the flavonoid biosynthesis pathway involved in flower color. In the present study, the effect of daminozide on the regulation of flower color was investigated. An herbaceous peony ( Pall.) cultivar 'Fenzhu Pan' was used as the material and treated with daminozide. Color parameters of petals were measured; flavonoid composition and content as well as flavonoid biosynthetic gene expression were monitored. The results showed that daminozide treatment caused less red coloration in herbaceous peony. The content of total anthocyanins was decreased by 23%, and the content of total anthoxanthins was increased by 8%; thus, it appeared that less red coloration in daminozide-treated flowers was associated with restricted anthocyanin accumulation. Furthermore, the gene expression patterns of flavonoid 3'-hydroxylase, dihydroflavonol 4-reductase and anthocyanidin synthase were lower after daminozide treatment, which could decrease anthocyanin accumulation and lead to less red coloration in flowers. These findings would improve our understanding of daminozide regulation of flower color in herbaceous peony.
丁酰肼是一种植物生长延缓剂，它能抑制赤霉素生物合成途径中依赖2-氧代戊二酸的双加氧酶活性，但很少有研究关注其对参与花色形成的类黄酮生物合成途径的影响。在本研究中，对丁酰肼对花色调控的作用进行了研究。以芍药（Pall.）品种‘粉珠盘’为材料，用丁酰肼进行处理。测定花瓣的颜色参数；监测类黄酮的组成、含量以及类黄酮生物合成基因的表达。结果表明，丁酰肼处理使芍药的红色变浅。总花青素含量降低了23%，总黄酮含量增加了8%；因此，丁酰肼处理的花朵红色变浅似乎与花青素积累受限有关。此外，丁酰肼处理后，类黄酮3'-羟化酶、二氢黄酮醇4-还原酶和花青素合酶的基因表达模式降低，这可能会减少花青素的积累，导致花朵红色变浅。这些发现将增进我们对丁酰肼调控芍药花色的理解。
