清除鼠贾第虫感染需要辅助/诱导性T淋巴细胞。
Clearance of Giardia muris infection requires helper/inducer T lymphocytes.
作者信息
Heyworth M F, Carlson J R, Ermak T H
出版信息
J Exp Med. 1987 Jun 1;165(6):1743-8. doi: 10.1084/jem.165.6.1743.
Abstract
The aim of this work was to identify the T cell subset (helper/inducer or cytotoxic/suppressor) that plays a major part in the clearance of Giardia muris infection. BALB/c mice were selectively depleted of helper/inducer (Th/i) or cytotoxic/suppressor (Tc/s) T cells, by treatment with rat IgG monoclonal antibodies directed against the Th/i cell surface antigen L3T4, or against the Tc/s cell surface antigen Ly-2, and were infected with G. muris cysts. While mice depleted of Tc/s cells cleared G. muris infection at a normal rate, mice depleted of Th/i cells did not clear the infection. Depletion of the relevant T cell subset was confirmed by flow cytometry and by immunohistochemistry. The data indicate that helper/inducer T cells, but not cytotoxic T cells, are of major importance for elimination of G. muris from the mouse intestine.
摘要
这项工作的目的是确定在清除鼠贾第虫感染中起主要作用的T细胞亚群（辅助/诱导或细胞毒性/抑制）。通过用针对辅助/诱导（Th/i）细胞表面抗原L3T4或细胞毒性/抑制（Tc/s）细胞表面抗原Ly-2的大鼠IgG单克隆抗体处理，选择性去除BALB/c小鼠的辅助/诱导（Th/i）或细胞毒性/抑制（Tc/s）T细胞，然后用鼠贾第虫囊肿感染它们。虽然去除Tc/s细胞的小鼠以正常速率清除了鼠贾第虫感染，但去除Th/i细胞的小鼠没有清除感染。通过流式细胞术和免疫组织化学证实了相关T细胞亚群的去除。数据表明，辅助/诱导T细胞而非细胞毒性T细胞对于从小鼠肠道中清除鼠贾第虫至关重要。
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