Kumar Ashok, Kim In-Won, Patel Sanjay K S, Lee Jung-Kul
Department of Chemical Engineering, Konkuk University, 1 Hwayang-Dong, Gwangjin-Gu, Seoul, 05029 Republic of Korea.
Indian J Microbiol. 2018 Mar;58(1):100-104. doi: 10.1007/s12088-017-0700-2. Epub 2017 Dec 30.
In the present study, a method for easy and rapid synthesis of lipase nanohybrids was evaluated using cobalt chloride as an encapsulating agent. The synthesized nanohybrids exhibited higher activity (181%) compared to free lipase and improved catalytic properties at higher temperature and in harsh conditions. The nanohybrids retained 84% of their residual activity at 25 °C after 10 days. In addition, these nanohybrids also exhibited high storage stability and reusability. Collectively, the synthesis of carrier-free immobilized biocatalysts was performed rapidly within 24 h at 4 °C. Their high reusability and catalytic activities highlight the broad applicability of this method for catalysis in organic and aqueous media.
在本研究中，评估了一种使用氯化钴作为包封剂轻松快速合成脂肪酶纳米杂化物的方法。与游离脂肪酶相比，合成的纳米杂化物表现出更高的活性（181%），并在较高温度和苛刻条件下具有改善的催化性能。纳米杂化物在25°C下放置10天后仍保留其84%的残余活性。此外，这些纳米杂化物还表现出高储存稳定性和可重复使用性。总体而言，在4°C下24小时内即可快速完成无载体固定化生物催化剂的合成。它们的高可重复使用性和催化活性突出了该方法在有机和水性介质中催化的广泛适用性。
