Kim Hyo-Jung, Dasagrandhi Chakradhar, Kim Song-Hee, Kim Bo-Geum, Eom Sung-Hwan, Kim Young-Mog
1Department of Food Science and Technology, Pukyong National University, Busan, 48547 Korea.
2Marine-Integrated Bionics Research Center, Pukyong National University, Busan, 48513 Korea.
Indian J Microbiol. 2018 Mar;58(1):105-108. doi: 10.1007/s12088-017-0693-x. Epub 2017 Nov 21.
(LM) is an important food borne pathogen responsible for listeriosis. Further, LM is an etiological agent associated with life threatening conditions like meningitis and encephalitis. Biofilm forming and drug resistant LM may potentially become difficult to treat infections and hence effective controlling measures are required to prevent LM infections. In view of this, the present study evaluated an anti-listerial potential of edible brown seaweed, , by disc diffusion and micro-dilution methods. The results of the present study suggested that the anti-listerial activity of various phlorotannins isolated form were in the range of 16-256 µg/ml. Among the phlorotannins isolated, fucofuroeckol-A (FAA) exhibited the highest anti-listerial potential (MIC range 16-32 µg/ml) against LM strains tested. Further, in checker board synergy assays, FFA-streptomycin combination exhibited significant synergy (fractional inhibitory concentration index, ∑FIC < 0.5) against aminoglycoside resistant clinical strains of LM. The results of the present study suggested the potential use of edible seaweed as a source of natural phlorotannins to control food borne pathogenic infections.
单核细胞增生李斯特菌（LM）是引起李斯特菌病的一种重要食源性病原体。此外，LM还是与脑膜炎和脑炎等危及生命的疾病相关的病原体。形成生物膜和具有耐药性的LM可能会导致感染难以治疗，因此需要有效的控制措施来预防LM感染。鉴于此，本研究通过纸片扩散法和微量稀释法评估了可食用褐藻的抗李斯特菌潜力。本研究结果表明，从该褐藻中分离出的各种间苯三酚单宁的抗李斯特菌活性在16 - 256微克/毫升范围内。在所分离的间苯三酚单宁中，岩藻呋喃酚A（FAA）对所测试的LM菌株表现出最高的抗李斯特菌潜力（最低抑菌浓度范围为16 - 32微克/毫升）。此外，在棋盘协同试验中，FFA - 链霉素组合对耐氨基糖苷类的临床LM菌株表现出显著的协同作用（分数抑菌浓度指数，∑FIC < 0.5）。本研究结果表明，可食用海藻有潜力作为天然间苯三酚单宁的来源用于控制食源性病原体感染。
