Li Ying, Zhang Shuang, He Mu, Zhang Yanchun, Fu Yanyan, Liang Hao, Jing Hongbo, Li Yindong, Ma Hongmei, Zhang Maojun
Shunyi District Center for Disease Control and Prevention, Beijing, China.
National Institute for Communicable Disease Control and Prevention, Beijing, China.
Front Microbiol. 2018 Jan 26;9:52. doi: 10.3389/fmicb.2018.00052. eCollection 2018.
Bacterial pathogens have been confirmed as the major cause of acute diarrhea among outpatients in China. In this study, 370 stool samples from the patients aged from 15 to 87 years old with diarrhea were collected over 12 months (from May 2016 to April 2017) in two hospitals in Shunyi, Beijing. Bacterial isolation was performed for the common enteric pathogens: , and for 370 samples. The filtration method was used for the isolation in this study. The prevalence and molecular characterization of the were investigated. The isolation ratio for , , , and was 7.0% (26/370), 6.2% (23/370), 0.3% (1/370), 7.3% (27/370), and 10.3% (38/370), respectively. Based on the isolation result, positive cases presented in almost every month of the whole year and the isolation ratio was the highest among the tested pathogens during October to March. There was no significant difference between genders of positive cases. More positive cases presented dehydration compared with those who were positive for . Twenty-six isolates were obtained in this study and 24 of these were The antibiotic susceptibility tests indicated that 83.3% (20/24) of the isolates exhibited resistance to three or more types of antibiotic. Twenty STs were identified for the 26 isolates and four novel STs were identified in this study. No clonal cluster was found among these isolates. This is the first study for isolated using the filtration method in China which indicated the infection might be seriously under-ascertained in the diarrheal patients in China.
细菌病原体已被确认为中国门诊患者急性腹泻的主要原因。在本研究中，于2016年5月至2017年4月的12个月期间，在北京顺义的两家医院收集了370份年龄在15至87岁之间腹泻患者的粪便样本。对常见肠道病原体进行细菌分离：对370份样本进行了[具体病原体]的分离。本研究采用过滤法进行[具体病原体]的分离。对[具体病原体]的流行情况和分子特征进行了调查。[具体病原体1]、[具体病原体2]、[具体病原体3]、[具体病原体4]和[具体病原体5]的分离率分别为7.0%（26/370）、6.2%（23/370）、0.3%（1/370）、7.3%（27/370）和10.3%（38/370）。根据分离结果，[具体病原体]阳性病例几乎出现在全年的每个月，且在10月至次年3月期间其分离率在检测的病原体中最高。[具体病原体]阳性病例的性别之间无显著差异。与[另一病原体]阳性患者相比，更多[具体病原体]阳性病例出现脱水。本研究共获得26株[具体病原体]分离株，其中24株为[具体类型]。抗生素敏感性试验表明，83.3%（20/24）的分离株对三种或更多类型的抗生素耐药。对26株[具体病原体]分离株鉴定出20种序列型（STs），本研究中鉴定出4种新的STs。在这些分离株中未发现克隆簇。这是中国首次使用过滤法分离[具体病原体]的研究，表明在中国腹泻患者中[具体病原体]感染可能严重漏报。
