Benner Bruce A, Schantz Michele M, Powers Carissa D, Schleicher Rosemary L, Camara Johanna E, Sharpless Katherine E, Yen James H, Sniegoski Lorna T
Chemical Sciences Division, National Institute of Standards and Technology, 100 Bureau Drive, Gaithersburg, MD, 20899, USA.
Division of Laboratory Sciences, National Center for Environmental Health, Centers for Disease Control and Prevention, 4770 Buford Hwy, Atlanta, GA, 30341, USA.
Anal Bioanal Chem. 2018 Mar;410(9):2321-2329. doi: 10.1007/s00216-017-0841-5. Epub 2018 Feb 13.
Dietary fatty acids can be both beneficial and detrimental to human health depending on the degree and type of saturation. Healthcare providers and research scientists monitor the fatty acid content of human plasma and serum as an indicator of health status and diet. In addition, both the Centers for Disease Control & Prevention (CDC) and the National Institutes of Health - Office of Dietary Supplements are interested in circulating fatty acids (FAs) because they may be predictive of coronary heart disease. The National Institute of Standards and Technology (NIST) provides a wide variety of reference materials (RMs) and Standard Reference Materials® (SRM®s) including blood, serum, plasma, and urine with values assigned for analytes of clinical interest. NIST SRM 2378 Fatty Acids in Frozen Human Serum was introduced in 2015 to help validate methods used for the analysis of FAs in serum, and consists of three different pools of serum acquired from (1) healthy donors who had taken fish oil dietary supplements (at least 1000 mg per day) for at least one month (level 1 material), (2) healthy donors who had taken flaxseed oil dietary supplements (at least 1000 mg per day) for at least one month (level 2 material), and (3) healthy donors eating "normal" diets who had not taken dietary supplements containing fish or plant oils (level 3 material). The use of dietary supplements by donors provided SRMs with natural endogenous ranges of FAs at concentrations observed in human populations. Results from analyses using two methods at NIST, including one involving a novel microwave-assisted acid hydrolysis procedure, and one at the CDC are presented here. These results and their respective uncertainties were combined to yield certified values with expanded uncertainties for 12 FAs and reference values with expanded uncertainties for an additional 18 FAs.
膳食脂肪酸对人体健康的影响既可能有益，也可能有害，这取决于其饱和程度和类型。医疗保健提供者和科研人员会监测人体血浆和血清中的脂肪酸含量，以此作为健康状况和饮食的一个指标。此外，疾病控制与预防中心（CDC）以及国立卫生研究院膳食补充剂办公室都对循环脂肪酸（FAs）感兴趣，因为它们可能是冠心病的预测指标。美国国家标准与技术研究院（NIST）提供了各种各样的参考物质（RMs）和标准参考物质（SRM®s），包括血液、血清、血浆和尿液，这些物质都已指定了临床相关分析物的值。NIST SRM 2378冷冻人血清中的脂肪酸于2015年推出，旨在帮助验证用于分析血清中脂肪酸的方法，它由从以下三类人群获取的三种不同血清池组成：（1）每天服用至少1000毫克鱼油膳食补充剂至少一个月的健康捐赠者（1级物质）；（2）每天服用至少1000毫克亚麻籽油膳食补充剂至少一个月的健康捐赠者（2级物质）；（3）未服用含鱼油或植物油膳食补充剂且饮食“正常”的健康捐赠者（3级物质）。捐赠者使用膳食补充剂为标准参考物质提供了在人群中观察到的脂肪酸自然内源性浓度范围。本文展示了在美国国家标准与技术研究院使用两种方法的分析结果，其中一种方法涉及一种新型微波辅助酸水解程序，另一种是疾病控制与预防中心的方法。将这些结果及其各自的不确定度相结合，得出了12种脂肪酸的具有扩展不确定度的认证值以及另外18种脂肪酸的具有扩展不确定度的参考值。
