Schantz Michele M, Bedner Mary, Long Stephen E, Molloy John L, Murphy Karen E, Porter Barbara J, Putzbach Karsten, Rimmer Catherine A, Sander Lane C, Sharpless Katherine E, Thomas Jeanice B, Wise Stephen A, Wood Laura J, Yen James H, Yarita Takashi, NguyenPho Agnes, Sorenson Wendy R, Betz Joseph M
Analytical Chemistry Division, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.
Anal Bioanal Chem. 2008 Oct;392(3):427-38. doi: 10.1007/s00216-008-2297-0. Epub 2008 Aug 2.
As part of a collaboration with the National Institutes of Health's Office of Dietary Supplements and the Food and Drug Administration's Center for Drug Evaluation and Research, the National Institute of Standards and Technology has developed two standard reference materials (SRMs) representing different forms of saw palmetto (Serenoa repens), SRM 3250 Serenoa repens fruit and SRM 3251 Serenoa repens extract. Both of these SRMs have been characterized for their fatty acid and phytosterol content. The fatty acid concentration values are based on results from gas chromatography with flame ionization detection (GC-FID) and mass spectrometry (GC/MS) analysis while the sterol concentration values are based on results from GC-FID and liquid chromatography with mass spectrometry analysis. In addition, SRM 3250 has been characterized for lead content, and SRM 3251 has been characterized for the content of beta-carotene and tocopherols. SRM 3250 (fruit) has certified concentration values for three phytosterols, 14 fatty acids as triglycerides, and lead along with reference concentration values for four fatty acids as triglycerides and 16 free fatty acids. SRM 3251 (extract) has certified concentration values for three phytosterols, 17 fatty acids as triglycerides, beta-carotene, and gamma-tocopherol along with reference concentration values for three fatty acids as triglycerides, 17 fatty acids as free fatty acids, beta-carotene isomers, and delta-tocopherol and information values for two phytosterols. These SRMs will complement other reference materials currently available with concentrations for similar analytes and are part of a series of SRMs being developed for dietary supplements.
作为与美国国立卫生研究院膳食补充剂办公室以及美国食品药品监督管理局药品评价和研究中心合作的一部分，美国国家标准与技术研究院已开发出两种代表不同形式锯棕榈（锯叶棕）的标准参考物质（SRM），即SRM 3250锯叶棕果实和SRM 3251锯叶棕提取物。这两种SRM均已对其脂肪酸和植物甾醇含量进行了表征。脂肪酸浓度值基于气相色谱 - 火焰离子化检测（GC - FID）和质谱（GC/MS）分析结果，而甾醇浓度值基于GC - FID和液相色谱 - 质谱分析结果。此外，SRM 3250已对铅含量进行了表征，SRM 3251已对β - 胡萝卜素和生育酚含量进行了表征。SRM 3250（果实）具有三种植物甾醇、14种作为甘油三酯的脂肪酸以及铅的认证浓度值，还有四种作为甘油三酯的脂肪酸和16种游离脂肪酸的参考浓度值。SRM 3251（提取物）具有三种植物甾醇、17种作为甘油三酯的脂肪酸、β - 胡萝卜素和γ - 生育酚的认证浓度值，以及三种作为甘油三酯的脂肪酸、17种游离脂肪酸、β - 胡萝卜素异构体和δ - 生育酚的参考浓度值，还有两种植物甾醇的信息值。这些SRM将补充目前可获得的具有类似分析物浓度的其他参考物质，并且是正在为膳食补充剂开发的一系列SRM的一部分。
