Unité Mixte de Recherche Centre national de la Recherche Scientifique 7276, INSERM U1262 Contrôle de la Réponse Immune B et Lymphoproliférations, Université de Limoges, Limoges, France.
Blood Adv. 2018 Feb 13;2(3):252-262. doi: 10.1182/bloodadvances.2017014423.
The immunoglobulin heavy chain (IgH) 3' regulatory region (3'RR) superenhancer controls B2 B-cell IgH transcription and cell fate at the mature stage but not early repertoire diversity. B1 B cells represent a small percentage of total B cells differing from B2 B cells by several points such as precursors, development, functions, and regulation. B1 B cells act at the steady state to maintain homeostasis in the organism and during the earliest phases of an immune response, setting them at the interface between innate and acquired immunity. We investigated the role of the 3'RR superenhancer on B1 B-cell fate. Similar to B2 B cells, the 3'RR controls μ transcription and cell fate in B1 B cells. In contrast to B2 B cells, 3'RR deletion affects B1 B-cell late repertoire diversity. Thus, differences exist for B1 and B2 B-cell 3'RR control during B-cell maturation. For the first time, these results highlight the contribution of the 3'RR superenhancer at this interface between innate and acquired immunity.
免疫球蛋白重链（IgH）3'调控区（3'RR）超级增强子控制 B2 B 细胞 IgH 转录和成熟阶段的细胞命运，但不控制早期库多样性。B1 B 细胞是总 B 细胞的一小部分，与 B2 B 细胞在多个方面存在差异，如前体、发育、功能和调节。B1 B 细胞在稳态下发挥作用，以维持生物体的内稳态，并在免疫反应的早期阶段发挥作用，使它们处于先天免疫和获得性免疫之间的界面。我们研究了 3'RR 超级增强子对 B1 B 细胞命运的作用。与 B2 B 细胞相似，3'RR 控制 B1 B 细胞中的 μ 转录和细胞命运。与 B2 B 细胞不同的是，3'RR 缺失影响 B1 B 细胞晚期库多样性。因此，B 细胞成熟过程中 B1 和 B2 B 细胞 3'RR 控制存在差异。这些结果首次强调了 3'RR 超级增强子在先天免疫和获得性免疫之间的这个界面中的作用。
