Bisgaard H, Kristensen J K, Sondergaard J
J Invest Dermatol. 1986 Mar;86(3):275-8. doi: 10.1111/1523-1747.ep12285417.
By combining laser-Doppler velocimetry with reactive hyperemia, induced by arterial occlusion of 4-min duration we have established a simple and atraumatic new ranking technique for assessing steroid potency. The experiments were performed in 19 healthy volunteers. Budesonide (Preferid) and placebo (vehicle) were applied under occlusion on the flexor aspect of the forearm for 2, 24, and 48 h. Blood flow was measured with a laser-Doppler flowmeter before and after arterial occlusion with a blood pressure cuff on the upper arm. The induced reactive hyperemia reaction was measured planimetrically on the registered curves. The steroid preparation caused a significant reduction of the hyperemia closely related to the application time. The reduction of the hyperemia was confirmed using the atraumatic 133Xe washout technique. In another group, hydrocortisone, hydrocortisone-butyrate (Locoid), budesonide (Preferid), and klobetasol (Dermovat) applied under occlusion for 1 h were compared. A significant dose-response relation corresponding to the expected rank-order efficacy was revealed. In 10 experiments, pretreatment with 50 mg indomethacin i.v. resulted in a significant reduction of the hyperemia reaction. As a hypothesis we therefore suggest that the reactive hyperemia reaction amplifies the vascular events and unmasks steroid-induced inhibition of the synthesis of arachidonic acid metabolites.
通过将激光多普勒测速技术与由持续4分钟的动脉闭塞诱导的反应性充血相结合，我们建立了一种简单且无创的新分级技术来评估类固醇的效力。实验在19名健康志愿者身上进行。将布地奈德（普米克令舒）和安慰剂（赋形剂）在闭塞状态下涂于前臂屈侧2小时、24小时和48小时。使用激光多普勒流量计在上臂使用血压袖带进行动脉闭塞前后测量血流量。在记录曲线上用平面测量法测量诱导的反应性充血反应。类固醇制剂导致与应用时间密切相关的充血显著减少。使用无创的133Xe洗脱技术证实了充血的减少。在另一组实验中，比较了在闭塞状态下应用1小时的氢化可的松、丁酸氢化可的松（乐肤液）、布地奈德（普米克令舒）和氯倍他索（ Dermovat）。揭示了与预期的效价顺序相对应的显著剂量反应关系。在10次实验中，静脉注射50毫克吲哚美辛预处理导致充血反应显著降低。因此，作为一种假设，我们认为反应性充血反应放大了血管事件，并揭示了类固醇诱导的花生四烯酸代谢物合成抑制。
