College of Animal Science and Technology, No. 6, Tongwei Road, Xuanwu District, Nanjing, Jiangsu 210095, P. R. China.
College of Veterinary Medicine, Nanjing Agricultural University, No. 6, Tongwei Road, Xuanwu District, Nanjing, Jiangsu 210095, P. R. China.
Poult Sci. 2018 Apr 1;97(4):1209-1219. doi: 10.3382/ps/pex408.
The object of this study was to investigate the effect of curcumin on modulating the glutathione (GSH)-related antioxidant enzymes and antioxidant responses via NF-E2-related factor 2 (Nrf2) signaling pathway in heat-stressed broiler chickens. A total of 400 one-day-old male Arbor Acres broiler chicks was reared in an environmentally controlled room. At 21 d, broiler chicks were divided into 5 treatment groups and were fed one of 4 diets under 2 temperature conditions: 22°C + a basal diet (CON treatment); 34°C for 8 h (0900-1700) + a basal diet supplemented with 0, 50, 100, or 200 mg/kg curcumin (HS, CMN1, CMN2, and CMN3 treatments, respectively). The heat treatment lasted for 20 consecutive days. The results showed that heat stress significantly increased (P < 0.05) the weekly rectal temperature and average head and feet temperature. Compared to the HS treatment, feed conversion was significantly decreased (P < 0.05) in CMN1 and CMN2 treatments. CMN1 administration significantly improved (P < 0.05) the pH24 of muscle. The abnormal changes of serum malonaldehyde and corticosterone concentrations were prevented (P < 0.05) by curcumin. Mitochondrial GSH concentration in the liver was significantly increased (P < 0.05) in CMN1 and CMN2 treatments compared with the HS treatment. The CMN1, CMN2, and CMN3 supplementations significantly increased (P < 0.05) γ-GCL, GSH-Px, and GST activities. Curcumin significantly increased (P < 0.05) the expression of Nrf2, HO-1, and γ-GCLc in the liver as compared to the CON diet. The expression of Cu/ZnSOD and CAT were increased (P < 0.05) by feeding CMN2, respectively, as compared to the HS treatment. It was concluded that curcumin supplementation enhanced the resistance of broilers to heat stress, as evidenced by reversing the FC, increasing the GSH content and GSH-related enzyme activities, and inducing the expression of Nrf2 and Nrf2-mediated phase II detoxifying enzyme genes.
本研究旨在探讨姜黄素通过核因子 E2 相关因子 2（Nrf2）信号通路对热应激肉鸡谷胱甘肽（GSH）相关抗氧化酶和抗氧化反应的调节作用。将 400 只 1 日龄雄性 Arbor Acres 肉鸡在环境控制室内饲养。21 日龄时，肉鸡被分为 5 个处理组，在 2 种温度条件下分别饲喂 4 种日粮中的 1 种：22°C+基础日粮（CON 处理）；34°C 8 小时（0900-1700）+基础日粮中添加 0、50、100 或 200mg/kg 姜黄素（HS、CMN1、CMN2 和 CMN3 处理，分别）。热应激持续 20 天。结果表明，热应激显著增加（P < 0.05）肉鸡每周直肠温度和平均头部及脚部温度。与 HS 处理相比，CMN1 和 CMN2 处理的饲料转化率显著降低（P < 0.05）。CMN1 处理显著提高（P < 0.05）肌肉 pH24。姜黄素可防止血清丙二醛和皮质酮浓度的异常变化（P < 0.05）。与 HS 处理相比，CMN1 和 CMN2 处理肝脏线粒体 GSH 浓度显著升高（P < 0.05）。CMN1、CMN2 和 CMN3 补充剂显著增加（P < 0.05）γ-GCL、GSH-Px 和 GST 活性。与 CON 日粮相比，姜黄素显著增加（P < 0.05）肝脏中 Nrf2、HO-1 和 γ-GCLc 的表达。与 HS 处理相比，CMN2 分别增加（P < 0.05）Cu/ZnSOD 和 CAT 的表达。综上所述，姜黄素的添加增强了肉鸡对热应激的抵抗力，表现为逆转 FC，增加 GSH 含量和 GSH 相关酶活性，并诱导 Nrf2 和 Nrf2 介导的Ⅱ相解毒酶基因的表达。
