College of Animal Science, Yangtze University, Jingzhou, P.R. China.
State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, P.R. China.
Poult Sci. 2018 Feb 1;97(2):599-606. doi: 10.3382/ps/pex353.
This study aims to investigate the effects of epigallocatechin gallate (EGCG) on the growth performance and serum metabolic characteristics of heat-stressed broilers. A total of 192 14-day-old Arbor Acres broilers were divided into 4 groups with 6 replicates per group (8 chickens/cage). Thermoneutral group (Group TN) was fed the basal diet and maintained at 28°C for 24 h/d. The heat-stressed groups were housed at 35°C for 12 h/d and 28°C for 12 h/d and fed the basal diet supplemented with EGCG at 0, 300, and 600 mg/kg diet (Groups HS0, HS300, and HS600, respectively). The production performance and serum metabolic characteristics were analyzed at d 21, 28, and 35, respectively. At d 35 of age, heat stress reduced (P < 0.05) the body weight (BW), feed intake (FI) and the contents of serum total protein (TP) and glucose (GLU); inhibited (P < 0.05) alkaline phosphatase (ALP) activity; But increased (P < 0.05) the contents of uric acid (UA), cholesterol (CHOL), triglyceride (TG), and the activities of creatine kinase (CK), lactate dehydrogenase (LDH), aspartate aminotransferase (AST). Heat-stressed chickens fed EGCG exhibited a linear increase (P < 0.05) in BW, FI, the levels of serum TP, GLU, and ALP activity; and linear decrease (P < 0.05) in the contents of serum UA, CHOL, and TG, as well as the activities of LDH, CK, and AST. Heat stress also reduced (P < 0.05) the activities of serum glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) on d 35 and increased (P < 0.05) the GSH-Px and SOD activity on d 21 and malondialdehyde (MDA) contents. There was a linear increase (P < 0.05) in activities of GSH-Px, SOD and CAT at 35 d of age, and linear decreased (P < 0.05) in MDA contents. In conclusion, EGCG can improve the growth performance of broilers by enhancing antioxidant property and alleviating oxidant damage caused by heat stress.
本研究旨在探讨表没食子儿茶素没食子酸酯（EGCG）对热应激肉鸡生长性能和血清代谢特性的影响。将 192 只 14 日龄的阿伯丁·阿科克斯肉鸡分为 4 组，每组 6 个重复（每笼 8 只鸡）。常温组（TN 组）饲喂基础日粮，每天 28°C 饲养 24 h。热应激组每天 12 h 35°C 饲养，12 h 28°C 饲养，并在基础日粮中添加 0、300 和 600 mg/kg 日粮的 EGCG（分别为 HS0、HS300 和 HS600 组）。分别在第 21、28 和 35 天分析生产性能和血清代谢特性。35 日龄时，热应激降低了（P < 0.05）体重（BW）、采食量（FI）和血清总蛋白（TP）和葡萄糖（GLU）含量；抑制（P < 0.05）碱性磷酸酶（ALP）活性；但增加（P < 0.05）尿酸（UA）、胆固醇（CHOL）、甘油三酯（TG）和肌酸激酶（CK）、乳酸脱氢酶（LDH）、天门冬氨酸转氨酶（AST）的含量。热应激鸡饲喂 EGCG 后，BW、FI、血清 TP、GLU 和 ALP 活性呈线性增加（P < 0.05）；血清 UA、CHOL 和 TG 含量及 LDH、CK 和 AST 活性呈线性下降（P < 0.05）。热应激还降低了（P < 0.05）血清谷胱甘肽过氧化物酶（GSH-Px）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）在第 35 天的活性，并增加了（P < 0.05）第 21 天和丙二醛（MDA）含量的 GSH-Px 和 SOD 活性。第 35 天，GSH-Px、SOD 和 CAT 的活性呈线性增加（P < 0.05），MDA 含量呈线性下降（P < 0.05）。综上所述，EGCG 通过增强抗氧化性能和缓解热应激引起的氧化损伤，可提高肉鸡的生长性能。
