纳米孔测序揭示 IncF 质粒上两个基因的共存。
Coexistence of Two Genes on an IncF Plasmid as Revealed by Nanopore Sequencing.
机构信息
Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China.
Division of Infectious Diseases, State Key Laboratory of Biotherapy, Chengdu, China.
出版信息
Antimicrob Agents Chemother. 2018 Apr 26;62(5). doi: 10.1128/AAC.00110-18. Print 2018 May.
Abstract
In a carbapenem-resistant clinical isolate of sequence type 167, two copies of were found on a 144,225-bp IncF self-transmissible plasmid of the F36:A4:B type. Both genes were located in 11,065-bp regions flanked by two copies of IS The two regions were identical in sequence but were present at different locations on the plasmid, suggesting a duplication of the same region. This study highlights the complex genetic contexts of .
摘要
在一株 167 型序列的耐碳青霉烯临床分离株中，发现 2 个 基因位于一个 144225bp 的 F36:A4:B 型 F 质粒上，该质粒可自我传递。两个 基因位于由 2 个 IS 侧翼的 11065bp 区域内，这两个区域的序列完全相同，但位于质粒的不同位置，提示该区域发生了重复。本研究强调了 的复杂遗传背景。
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