Ha Do Thi, Long Phung Thanh, Hien Tran Thi, Tuan Dao Trong, An Nguyen Thi Thuy, Khoi Nguyen Minh, Van Oanh Ha, Hung Tran Manh
Vietnam National Institute of Medicinal Materials, 3B Quangtrung, Hanoi, Vietnam.
Department of Experimental Medical Science, Faculty of Medicine, Lund University, BMC D12, 221 84, Lund, Sweden.
Chem Cent J. 2018 Feb 13;12(1):14. doi: 10.1186/s13065-018-0386-5.
Vitis heyneana is widely distributed in the north of Vietnam, it has been used in Vietnamese traditional medicine as an agent for treatment of arthritis, bronchitis, carbuncles and inflammatory conditions, and menstrual irregularities. However, this plant has not been investigated in phytochemical constituents and biological effects, especially in the anti-inflammatory property.
Bioassay-guided fractionation of the EtOAc soluble fraction from the aerial part of Vitis heyneana resulted in the isolation of a series of oligostilbenoids as piceid (1), 2-r-viniferin (2), betulifol A (3), vitisinol C (4), (-)-trans-ε-viniferin (5), α-viniferin (6), shoreaketon (7), amurensin B (8), vitisinol B (9), and cis-vitisin B (10). Compound 5 showed the most potent inhibitory activities by suppressing LPS-induced COX-2 expression and PGE2 production. This compound exhibited significantly reduced LPS-induced nitric oxide (NO) release in a dose-dependent manner. These effects are accompanied with the inhibition of transcription factor NF-κB activation.
The results suggested that trans-ε-viniferin exerts anti-inflammatory effects via suppression the NF-κB activation in RAW 264.7 cells.
越南蛇葡萄广泛分布于越南北部，在越南传统医学中被用作治疗关节炎、支气管炎、痈和炎症性疾病以及月经不调的药物。然而，该植物的植物化学成分和生物学效应，尤其是抗炎特性尚未得到研究。
对越南蛇葡萄地上部分的乙酸乙酯可溶部分进行生物活性导向分离，得到了一系列低聚芪类化合物，如白藜芦醇（1）、2-r-葡萄素（2）、桦叶醇A（3）、葡萄素醇C（4）、（-）-反式-ε-葡萄素（5）、α-葡萄素（6）、海滨酮（7）、山葡萄素B（8）、葡萄素醇B（9）和顺式葡萄素B（10）。化合物5通过抑制脂多糖（LPS）诱导的环氧化酶-2（COX-2）表达和前列腺素E2（PGE2）生成，显示出最有效的抑制活性。该化合物以剂量依赖性方式显著降低LPS诱导的一氧化氮（NO）释放。这些作用伴随着转录因子核因子κB（NF-κB）激活的抑制。
结果表明，反式-ε-葡萄素通过抑制RAW 264.7细胞中的NF-κB激活发挥抗炎作用。
