Abbas Ozan Luay, Özatik Orhan, Gönen Zeynep Burçin, Öğüt Serdal, Özatik Fikriye Yasemin, Salkın Hasan, Musmul Ahmet
Ahi Evran University, Faculty of Medicine, Department of Plastic, Reconstructive and Aesthetic Surgery, Kırşehir, Turkey.
Dumlupınar University, Faculty of Medicine, Department of Histology and Embryology, Kütahya, Turkey.
J Invest Surg. 2019 Sep;32(6):477-490. doi: 10.1080/08941939.2018.1433254. Epub 2018 Feb 14.
: The implantation of mesenchymal stem cells (MSCs) has been shown to exert benefits for the survival of the zone-of-stasis. However, the clinical experience indicates the importance of selecting the right source and type of stem cells. Therefore, we planned the current study to perform a quantitative comparison of MSCs isolated from three different sources to provide information useful in selection of the optimal source and to see whether critical mechanisms are conserved between different populations. : The protective effects of MSCs derived from bone marrow, adipose tissue and dental pulp were compared in a rat model of thermal trauma. The stasis zones were evaluated 72 h after the burn using histochemistry, immunohistochemistry and biochemistry. Gross evaluation of burn wounds revealed that the differences between the mean percentages of the calculated necrotic areas weren't statistically significant. Semi-quantitative grading of the histopathological findings revealed that there were no significant differences between damage scores. Immunohistochemical assessment of apoptotic and necrotic cell deaths revealed that the differences between the mean numbers of apoptotic and necrotic cells weren't statistically significant. Myeloperoxidase activity was found to be significantly lower in the adipose tissue group. Biochemical and immunohistochemical assessment of tissue malondialdehyde revealed that the differences between the groups weren't statistically significant. Finally, the number of neo-vessels in the dental pulp group was found to be significantly higher. Our findings suggest that bone marrow, adipose tissue and dental pulp may serve as a universal donor MSC source for the prevention of burn wound progression.
间充质干细胞（MSCs）的植入已被证明对淤滞区的存活有益。然而，临床经验表明选择正确的干细胞来源和类型很重要。因此，我们开展了本研究，对从三种不同来源分离的MSCs进行定量比较，以提供有助于选择最佳来源的信息，并观察不同群体之间关键机制是否保守。：在热创伤大鼠模型中比较了源自骨髓、脂肪组织和牙髓的MSCs的保护作用。烧伤72小时后，使用组织化学、免疫组织化学和生物化学方法评估淤滞区。烧伤创面的大体评估显示，计算出的坏死面积平均百分比之间的差异无统计学意义。组织病理学结果的半定量分级显示，损伤评分之间无显著差异。对凋亡和坏死细胞死亡的免疫组织化学评估显示，凋亡和坏死细胞的平均数量之间的差异无统计学意义。发现脂肪组织组的髓过氧化物酶活性显著较低。对组织丙二醛的生化和免疫组织化学评估显示，各组之间的差异无统计学意义。最后，发现牙髓组的新血管数量显著更高。我们的研究结果表明，骨髓、脂肪组织和牙髓可能作为预防烧伤创面进展的通用供体MSCs来源。
