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铜-壳聚糖纳米颗粒的应用促进龙爪稷(Eleusine coracana Gaertn.)植株生长并诱导其对稻瘟病产生抗性。

Application of Copper-Chitosan Nanoparticles Stimulate Growth and Induce Resistance in Finger Millet (Eleusine coracana Gaertn.) Plants against Blast Disease.

作者信息

Sathiyabama Muthukrishnan, Manikandan Appu

机构信息

Department of Botany, Bharathidasan University , Tiruchirappalli, Tamil Nadu 620 024, India.

出版信息

J Agric Food Chem. 2018 Feb 28;66(8):1784-1790. doi: 10.1021/acs.jafc.7b05921. Epub 2018 Feb 20.

Abstract

Copper-chitosan nanoparticle (CuChNp) was synthesized and used to study its effect on finger millet plant as a model plant system. Our objective was to explore the efficacy of CuChNp application to control blast disease of finger millet. CuChNp was applied to finger millet either as a foliar spray or as a combined application (involving seed coat and foliar spray). Both the application methods enhanced growth profile of finger millet plants and increased yield. The increased yield was nearly 89% in combined application method. Treated finger millet plants challenged with Pyricularia grisea showed suppression of blast disease development when compared to control. Nearly 75% protection was observed in the combined application of CuChNp to finger millet plants. In CuChNp treated finger millet plants, a significant increase in defense enzymes was observed, which was detected both qualitatively and quantitatively. The suppression of blast disease correlates well with increased defense enzymes in CuChNp treated finger millet plants.

摘要

合成了铜-壳聚糖纳米颗粒(CuChNp),并将其用于研究其对作为模式植物系统的龙爪稷植株的影响。我们的目标是探索施用CuChNp防治龙爪稷稻瘟病的效果。CuChNp以叶面喷施或联合施用(包括种衣和叶面喷施)的方式施用于龙爪稷。两种施用方法均改善了龙爪稷植株的生长状况并提高了产量。联合施用方法下产量提高了近89%。与对照相比,用稻瘟病菌攻击处理过的龙爪稷植株显示出稻瘟病发展受到抑制。在将CuChNp联合施用于龙爪稷植株时观察到近75%的保护率。在经CuChNp处理的龙爪稷植株中,观察到防御酶显著增加,这在定性和定量方面均得到检测。在经CuChNp处理的龙爪稷植株中,稻瘟病的抑制与防御酶的增加密切相关。

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