Raks Victoria, Al-Suod Hossam, Buszewski Bogusław
1Interdisciplinary Centre of Modern Technologies, Nicolaus Copernicus University, 4 Wileńska Str., 87-100 Toruń, Poland.
3Department of Analytical Chemistry, Faculty of Chemistry, Taras Shevchenko National University of Kyiv, Volodymyrska Street, 64/13, Kyiv, 01601 Ukraine.
Chromatographia. 2018;81(2):189-202. doi: 10.1007/s10337-017-3405-0. Epub 2017 Sep 22.
Development of efficient methods for isolation and separation of biologically active compounds remains an important challenge for researchers. Designing systems such as organomineral composite materials that allow extraction of a wide range of biologically active compounds, acting as broad-utility solid-phase extraction agents, remains an important and necessary task. Selective sorbents can be easily used for highly selective and reliable extraction of specific components present in complex matrices. Herein, state-of-the-art approaches for selective isolation, preconcentration, and separation of biologically active compounds from a range of matrices are discussed. Primary focus is given to novel extraction methods for some biologically active compounds including cyclic polyols, flavonoids, and oligosaccharides from plants. In addition, application of silica-, carbon-, and polymer-based solid-phase extraction adsorbents and membrane extraction for selective separation of these compounds is discussed. Potential separation process interactions are recommended; their understanding is of utmost importance for the creation of optimal conditions to extract biologically active compounds including those with estrogenic properties.
开发高效的生物活性化合物分离和提纯方法仍然是研究人员面临的一项重大挑战。设计诸如有机矿物复合材料之类的系统，使其能够作为通用的固相萃取剂来萃取多种生物活性化合物，仍然是一项重要且必要的任务。选择性吸附剂可轻松用于从复杂基质中高度选择性且可靠地萃取特定成分。本文讨论了从一系列基质中选择性分离、预富集和提纯生物活性化合物的最新方法。主要重点是一些生物活性化合物的新型萃取方法，包括从植物中提取环状多元醇、黄酮类化合物和寡糖。此外，还讨论了基于二氧化硅、碳和聚合物的固相萃取吸附剂以及膜萃取在这些化合物选择性分离中的应用。文中推荐了潜在的分离过程相互作用；了解它们对于创造最佳条件以萃取包括具有雌激素特性的生物活性化合物至关重要。
