采用时间和空间分辨单光子计数探测器的荧光寿命显微镜。
Fluorescence lifetime microscopy with a time- and space-resolved single-photon counting detector.
作者信息
Michalet X, Siegmund O H W, Vallerga J V, Jelinsky P, Pinaud F F, Millaud J E, Weiss S
机构信息
Department of Chemistry & Biochemistry, University of California at Los Angeles 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.
Experimental Astrophysics Group, Space Sciences Laboratory University of California at Berkeley, 7 Gauss Way, Berkeley, CA 94720, USA.
出版信息
Proc SPIE Int Soc Opt Eng. 2006;6372. doi: 10.1117/12.686429. Epub 2006 Oct 25.
We have recently developed a wide-field photon-counting detector (the H33D detector) having high-temporal and high-spatial resolutions and capable of recording up to 500,000 photons per sec. Its temporal performance has been previously characterized using solutions of fluorescent materials with different lifetimes, and its spatial resolution using sub-diffraction objects (beads and quantum dots). Here we show its application to fluorescence lifetime imaging of live cells and compare its performance to a scanning confocal TCSPC approach. With the expected improvements in photocathode sensitivity and increase in detector throughput, this technology appears as a promising alternative to the current lifetime imaging solutions.
我们最近开发了一种具有高时间分辨率和高空间分辨率的宽场光子计数探测器（H33D探测器），每秒能够记录多达500,000个光子。其时间性能先前已通过使用具有不同寿命的荧光材料溶液进行了表征，其空间分辨率则使用了亚衍射物体（珠子和量子点）进行了表征。在这里，我们展示了它在活细胞荧光寿命成像中的应用，并将其性能与扫描共聚焦时间相关单光子计数（TCSPC）方法进行了比较。随着光阴极灵敏度的预期提高和探测器通量的增加，这项技术似乎是当前寿命成像解决方案的一个有前途的替代方案。
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