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用于生物荧光成像的光子计数H33D探测器

Photon-Counting H33D Detector for Biological Fluorescence Imaging.

作者信息

Michalet X, Siegmund O H W, Vallerga J V, Jelinsky P, Millaud J E, Weiss S

机构信息

Department of Chemistry & Biochemistry, University of California at Los Angeles, 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.

出版信息

Nucl Instrum Methods Phys Res A. 2006 Nov 1;567(1):133. doi: 10.1016/j.nima.2006.05.155.

DOI:10.1016/j.nima.2006.05.155

PMID:20151021

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2819128/

Abstract

We have developed a photon-counting High-temporal and High-spatial resolution, High-throughput 3-Dimensional detector (H33D) for biological imaging of fluorescent samples. The design is based on a 25 mm diameter S20 photocathode followed by a 3-microchannel plate stack, and a cross delay line anode. We describe the bench performance of the H33D detector, as well as preliminary imaging results obtained with fluorescent beads, quantum dots and live cells and discuss applications of future generation detectors for single-molecule imaging and high-throughput study of biomolecular interactions.

摘要

我们开发了一种用于荧光样本生物成像的光子计数高时间分辨率、高空间分辨率、高通量三维探测器（H33D）。该设计基于一个直径25毫米的S20光电阴极，其后是一个由三个微通道板组成的堆栈，以及一个交叉延迟线阳极。我们描述了H33D探测器的台面性能，以及使用荧光珠、量子点和活细胞获得的初步成像结果，并讨论了下一代探测器在单分子成像和生物分子相互作用高通量研究中的应用。