Suppr超能文献

Single-quantum dot imaging with a photon counting camera.

作者信息

Michalet X, Colyer R A, Antelman J, Siegmund O H W, Tremsin A, Vallerga J V, Weiss S

机构信息

Department of Chemistry & Biochemistry, UCLA, Los Angeles, CA, USA.

出版信息

Curr Pharm Biotechnol. 2009 Aug;10(5):543-58. doi: 10.2174/138920109788922100.

Abstract

The expanding spectrum of applications of single-molecule fluorescence imaging ranges from fundamental in vitro studies of biomolecular activity to tracking of receptors in live cells. The success of these assays has relied on progress in organic and non-organic fluorescent probe developments as well as improvements in the sensitivity of light detectors. We describe a new type of detector developed with the specific goal of ultra-sensitive single-molecule imaging. It is a wide-field, photon-counting detector providing high temporal and high spatial resolution information for each incoming photon. It can be used as a standard low-light level camera, but also allows access to a lot more information, such as fluorescence lifetime and spatio-temporal correlations. We illustrate the single-molecule imaging performance of our current prototype using quantum dots and discuss on-going and future developments of this detector.

摘要

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验