一种用于荧光显微镜和光谱学的空间和时间分辨单光子计数探测器。
A space- and time-resolved single photon counting detector for fluorescence microscopy and spectroscopy.
作者信息
Michalet X, Siegmund O H W, Vallerga J V, Jelinsky P, Millaud J E, Weiss S
机构信息
Department of Chemistry & Biochemistry, University of California at Los Angeles 607 Charles E. Young Drive E., Los Angeles, CA 90095, USA.
Experimental Astrophysics Group, Space Sciences Laboratory University of California at Berkeley, 7 Gauss Way, Berkeley, CA 94720, USA.
出版信息
Proc SPIE Int Soc Opt Eng. 2006 Feb;6092. doi: 10.1117/12.646482.
We have recently developed a wide-field photon-counting detector having high-temporal and high-spatial resolutions and capable of high-throughput (the H33D detector). Its design is based on a 25 mm diameter multi-alkali photocathode producing one photo electron per detected photon, which are then multiplied up to 10 times by a 3-microchannel plate stack. The resulting electron cloud is proximity focused on a cross delay line anode, which allows determining the incident photon position with high accuracy. The imaging and fluorescence lifetime measurement performances of the H33D detector installed on a standard epifluorescence microscope will be presented. We compare them to those of standard single-molecule detectors such as single-photon avalanche photodiode (SPAD) or electron-multiplying camera using model samples (fluorescent beads, quantum dots and live cells). Finally, we discuss the design and applications of future generation of H33D detectors for single-molecule imaging and high-throughput study of biomolecular interactions.
我们最近开发了一种具有高时间分辨率和高空间分辨率且能够实现高通量的宽场光子计数探测器（H33D探测器）。其设计基于一个直径25毫米的多碱光电阴极，每检测到一个光子就产生一个光电子，然后通过一个3微通道板堆栈将其放大10倍。产生的电子云被近贴聚焦在一个交叉延迟线阳极上，这使得能够高精度地确定入射光子的位置。将展示安装在标准落射荧光显微镜上的H33D探测器的成像和荧光寿命测量性能。我们使用模型样品（荧光微球、量子点和活细胞）将它们与标准单分子探测器（如单光子雪崩光电二极管（SPAD）或电子倍增相机）的性能进行比较。最后，我们讨论了下一代用于单分子成像和生物分子相互作用高通量研究的H33D探测器的设计和应用。
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