Central European Institute of Technology (CEITEC), Masaryk University, Brno, Czech Republic.
Department of Comprehensive Cancer Care, Masaryk Memorial Cancer Institute, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Clin Chem Lab Med. 2018 May 24;56(6):869-879. doi: 10.1515/cclm-2017-0958.
Cerebrospinal fluid (CSF) is a body fluid that has many important functions and is in direct contact with the extracellular environment of the central nervous system (CNS). CSF serves as both the communication channel allowing the distribution of various substances among the CNS cells and the storage facility for the waste products these cells release. For these reasons, CSF is a potential source of diagnostic biomarkers of many CNS diseases, including brain tumors. Recent studies have revealed that CSF also contains circulating microRNAs (miRNAs), short non-coding RNAs that have been described as biomarkers in many cancers. However, CSF miRNAs are difficult to detect, which is why researchers face major challenges, including technological difficulties in its detection and its lack of standardization. Therefore, this review aims (i) to highlight the potential of CSF miRNAs as diagnostic, prognostic and predictive biomarkers in brain tumors, and (ii) to summarize technological approaches for detection of CSF miRNAs.
脑脊液（CSF）是一种具有许多重要功能的体液，与中枢神经系统（CNS）的细胞外环境直接接触。CSF 既是允许 CNS 细胞之间各种物质分布的通讯通道，也是这些细胞释放的废物的储存设施。出于这些原因，CSF 是许多 CNS 疾病（包括脑肿瘤）的诊断生物标志物的潜在来源。最近的研究表明，CSF 还含有循环 microRNAs（miRNAs），这是一种已被描述为许多癌症的生物标志物的短非编码 RNA。然而，CSF miRNAs 很难检测到，这就是为什么研究人员面临重大挑战，包括其检测的技术困难和缺乏标准化。因此，本综述旨在：（i）强调 CSF miRNAs 作为脑肿瘤诊断、预后和预测生物标志物的潜力；（ii）总结 CSF miRNAs 的检测技术方法。
