Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya 16/10, 117997 Moscow, Russia.
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Science, Miklukho-Maklaya 16/10, 117997 Moscow, Russia; Pirogov Russian National Research Medical University, 117997 Moscow, Russia.
Vaccine. 2018 Mar 14;36(12):1599-1605. doi: 10.1016/j.vaccine.2018.02.027. Epub 2018 Feb 15.
Vaccination against influenza is widely used to protect against seasonal flu epidemic although its effectiveness is debated. Here we performed deep quantitative T cell receptor repertoire profiling in peripheral blood of a healthy volunteer in response to trivalent subunit influenza vaccine. We did not observe significant rebuilding of peripheral blood T cell receptors composition in response to vaccination. However, we found several clonotypes in memory T cell fraction that were undetectable before the vaccination and had a maximum concentration at day 45 after vaccine administration. These cells were found in lower concentration in the course of repertoire monitoring for two years period. Our observation suggests a potential for recruitment of only a limited number of new T cells after each seasonal influenza vaccination.
接种流感疫苗被广泛用于预防季节性流感流行，尽管其效果存在争议。在这里，我们对一名健康志愿者接种三价亚单位流感疫苗后的外周血进行了深度定量 T 细胞受体库分析。我们没有观察到疫苗接种后外周血 T 细胞受体组成的显著重建。然而，我们发现记忆 T 细胞群中有几个克隆型在接种前无法检测到，并且在接种后第 45 天达到最高浓度。在两年的监测过程中，这些细胞的浓度较低。我们的观察表明，每次季节性流感疫苗接种后可能只能招募有限数量的新 T 细胞。
