Vaccinology & Immunotherapeutic Program, School of Public Health at the University of Saskatchewan, Canada; Vaccine & Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Canada.
Vaccine & Infectious Disease Organization-International Vaccine Centre (VIDO-InterVac), University of Saskatchewan, Canada.
Vaccine. 2018 Mar 14;36(12):1606-1613. doi: 10.1016/j.vaccine.2018.02.026. Epub 2018 Feb 14.
Swine influenza virus is endemic worldwide and it is responsible for significant economic losses to the swine industry. A vaccine that stimulates a rapid and long-lasting protective immune response to prevent this infection is highly sought. Poly[di(sodium carboxylatoethylphenoxy)-phosphazene (PCEP) has demonstrated adjuvant activity when formulated as part of multiple vaccines in mice and pigs. In this study we examined the magnitude and type of immune response induced in pigs vaccinated via the intramuscular or intradermal routes with inactivated swine influenza virus (SIV) H1N1 vaccine formulated with PCEP. Intradermal administration of PCEP-adjuvanted inactivated SIV vaccine stimulated significant anti-SIV antibody titres, increased neutralizing antibodies, and significantly reduced lung virus load with limited reduction of gross lung lesions after challenge with virulent H1N1 relative to control animals. These results indicate that PCEP may be effective as a vaccine adjuvant against swine influenza viruses in pigs and should be considered a potential candidate adjuvant for future swine intradermal influenza vaccines.
猪流感病毒在全球范围内流行，给养猪业造成了重大经济损失。因此，人们迫切需要一种能够刺激快速、持久的保护性免疫应答的疫苗来预防这种感染。聚[二(羧基乙酯基苯氧基)膦嗪]（PCEP）在作为多种疫苗的一部分进行配制时，在小鼠和猪中显示出佐剂活性。在这项研究中，我们通过肌肉内或皮内途径用含有 PCEP 的灭活猪流感病毒（SIV）H1N1 疫苗对猪进行免疫接种，检测了 PCEP 佐剂灭活 SIV 疫苗诱导的免疫应答的程度和类型。与对照动物相比，皮内给予 PCEP 佐剂的灭活 SIV 疫苗可刺激产生显著的抗 SIV 抗体滴度，增加中和抗体，并在攻毒后显著降低肺部病毒载量，同时肺部大体病变减少有限。这些结果表明，PCEP 可能是一种有效的猪流感病毒疫苗佐剂，可用于猪，并应被视为未来猪皮内流感疫苗的潜在候选佐剂。
