Baek Ki Ho, Heo Ye Seul, Park Joo Young, Kang Taemin, Lee Yee Eun, Lim Junghyun, Kim Seong Bong, Jo Cheorun
Department of Agricultural Biotechnology, Center for Food and Bioconvergence and Research Institute of Agriculture and Life Sciences, Seoul National University, Seoul 08826, Korea.
Surface Technology Division, Korea Institute of Material Sciences (KIMS), Changwon 51508, Korea.
Foods. 2020 Nov 18;9(11):1689. doi: 10.3390/foods9111689.
The key reactive species generated by non-thermal plasma bubbles for the inactivation of Typhimurium and the effects of organic matter on the inactivation efficacy were investigated. Plasma, which is primarily composed of ozone (O), was generated by dielectric barrier discharge and injected into a solution (400 mL) as a bubble. The surviving population of . Typhimurium decreased in proportion to the treatment time, resulting in a 5.29 log reduction after 5 min of treatment. Verification tests to specify key reactive species were conducted using an O destruction unit and reactive oxygen species scavengers. The results indicated that singlet oxygen (O) contributes substantially to the inactivation of . Typhimurium, and that the presence of superoxide anion radicals (O) from O is essential for the production of O. When a . Typhimurium suspension containing organic matter (final concentration: 0, 0.005, 0.05, 0.1, and 0.5 g/L), consisting of beef extract and peptone, was treated with plasma bubbles for 5, 10, 15, 20, 25, and 30 min, respectively, the potential of the plasma bubbles for inactivating . Typhimurium successfully was verified with longer contact time, despite organic matter attenuating the inactivation efficiency in a dose-dependent manner.
研究了非热等离子体气泡产生的关键反应性物种对鼠伤寒沙门氏菌的灭活作用以及有机物对灭活效果的影响。通过介质阻挡放电产生主要由臭氧（O）组成的等离子体，并以气泡形式注入到溶液（400 mL）中。鼠伤寒沙门氏菌的存活菌数随处理时间成比例减少，处理5分钟后减少了5.29个对数级。使用臭氧破坏装置和活性氧清除剂进行了确定关键反应性物种的验证试验。结果表明，单线态氧（O）对鼠伤寒沙门氏菌的灭活有很大贡献，并且来自臭氧的超氧阴离子自由基（O）的存在对于单线态氧的产生至关重要。当分别用等离子体气泡处理含有由牛肉提取物和蛋白胨组成的有机物（终浓度：0、0.005、0.05、0.1和0.5 g/L）的鼠伤寒沙门氏菌悬液5、10、15、20、25和30分钟时，尽管有机物以剂量依赖的方式降低了灭活效率，但通过更长的接触时间成功验证了等离子体气泡对鼠伤寒沙门氏菌的灭活潜力。
