Wang Xing, Deng Qing-Fang, Chen Hua-Guo, Zhou Xin
Key Laboratory for Information System of Mountain Areas and Protection of Ecological Environment, Guizhou Normal University, Guiyang 550001, China.
Guizhou Engineering Laboratory for Quality Control&Evaluation Technology of Medicine, Guizhou Normal University, Guiyang 550001, China.
Zhongguo Zhong Yao Za Zhi. 2017 Jun;42(12):2329-2333. doi: 10.19540/j.cnki.cjcmm.20170316.002.
Mori Fructus polysaccharides (MFPs) have been used as a source of therapeutic agents. The most promising activities of these biopolymers are their immunomodulation and anti-cancer effects. It was reported that polysaccharides were a potential drug against liver injury, but the hepatoprotective effect of MFPs was ambiguous. In this study, the fractionation of crude polysaccharides on DEAE-Cellulose 52 gave four fractions (MFP-1, MFP-2, MFP-2 and MFP-4). The results showed that the contents of carbohydrates were 75.3%, 83.7%, 79.1%, 74.3%, and the molecular weight of them were 112.2, 128.8, 199.5, 181.9 kDa. Monosaccharide component analysis indicated that MFP-1 was composed of galactose, glucose, arabinose, rhamnose and mannose with the molarity rate of 26.8∶20.4∶8.74∶5∶1; MFP-2 contained arabinose, galactose, rhamnose, glucose and galacturonic acid with the molarity rate of 34.2∶38.2∶8∶17.5∶15.1; MFP-3 was composed of galacturonic acid, galactose, glucose, rhamnose and arabinose with the molarity rate of 28.3∶22.6∶20.9∶18.6∶15.1; MFP-4 contained glucose, galactose, rhamnose, galacturonic acid, arabinose and glucuronic acid with the molarity rate of 47.4∶34.9∶36.1∶33.1∶19.9∶4.1. IR analysis's results indicated that MFP-3 and MFP-4 may be polysaccharides containing β-glycosides. The alcohol dehydrogenase (ADH) activity text showed that, all these four MFPs were found have a positive effect on the activity of ADH, with order: bifendate>MFP-3>MFP-1>MFP-2>tioprnin>MFP-4, and the MFP-3 had the highest activity and demonstrated outstanding hepatic protecting activity.
桑椹多糖（MFPs）已被用作治疗剂的来源。这些生物聚合物最有前景的活性是它们的免疫调节和抗癌作用。据报道，多糖是一种潜在的抗肝损伤药物，但MFPs的保肝作用尚不明确。在本研究中，粗多糖在DEAE-纤维素52上进行分级分离得到四个级分（MFP-1、MFP-2、MFP-3和MFP-4）。结果表明，它们的碳水化合物含量分别为75.3%、83.7%、79.1%、74.3%，分子量分别为112.2、128.8、199.5、181.9 kDa。单糖成分分析表明，MFP-1由半乳糖、葡萄糖、阿拉伯糖、鼠李糖和甘露糖组成，摩尔比为26.8∶20.4∶8.74∶5∶1；MFP-2含有阿拉伯糖、半乳糖、鼠李糖、葡萄糖和半乳糖醛酸，摩尔比为34.2∶38.2∶8∶17.5∶15.1；MFP-3由半乳糖醛酸、半乳糖、葡萄糖、鼠李糖和阿拉伯糖组成，摩尔比为28.3∶22.6∶20.9∶18.6∶15.1；MFP-4含有葡萄糖、半乳糖、鼠李糖、半乳糖醛酸、阿拉伯糖和葡萄糖醛酸，摩尔比为47.4∶34.9∶36.1∶33.1∶19.9∶4.1。红外分析结果表明，MFP-3和MFP-4可能是含有β-糖苷的多糖。乙醇脱氢酶（ADH）活性试验表明，这四种MFPs对ADH活性均有正向作用，顺序为：联苯双酯>MFP-3>MFP-1>MFP-2>硫普罗宁>MFP-4，且MFP-3活性最高，具有显著的保肝活性。
