Tang Lingling, Liu Jinjin, Zhu Linyun, Chen Qingge, Meng Ziyu, Sun Li, Hu Junsheng, Ni Zhenhua, Wang Xiongbiao
Department of Respiratory Medicine, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Central Laboratory, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
Front Pharmacol. 2018 Feb 2;9:60. doi: 10.3389/fphar.2018.00060. eCollection 2018.
Lung squamous cell carcinoma (LSCC) is a common histological lung cancer subtype, but unlike lung adenocarcinoma, limited therapeutic options are available for treatment. Curcumin, a natural compound, may have anticancer effects in various cancer cells, but how it may be used to treat LSCC has not been well studied. Here, we applied curcumin to a human NCI-H292 LSCC cell line to test anticancer effects and explored underlying potential mechanisms of action. Curcumin treatment inhibited NCI-H292 cell growth and increased FOXA2 expression in a time-dependent manner. FOXA2 expression was decreased in LSCC tissues compared with adjacent normal tissues and knockdown of FOXA2 increased NCI-H292 cells proliferation. Inhibition of cell proliferation by curcumin was attenuated by FOXA2 knockdown. Moreover inhibition of STAT3 pathways by curcumin increased FOXA2 expression in NCI-H292 cells whereas a STAT3 activator (IL-6) significantly inhibited curcumin-induced FOXA2 expression. Also, SOCS1 and SOCS3, negative regulators of STAT3 activity, were upregulated by curcumin treatment. Thus, curcumin inhibited human NCI-H292 cells growth by increasing FOXA2 expression via regulation of STAT3 signaling pathways.
肺鳞状细胞癌（LSCC）是一种常见的组织学肺癌亚型，但与肺腺癌不同，其治疗选择有限。姜黄素是一种天然化合物，可能对多种癌细胞具有抗癌作用，但它如何用于治疗LSCC尚未得到充分研究。在此，我们将姜黄素应用于人类NCI-H292 LSCC细胞系以测试其抗癌效果，并探索其潜在的作用机制。姜黄素处理以时间依赖性方式抑制NCI-H292细胞生长并增加FOXA2表达。与相邻正常组织相比，LSCC组织中FOXA2表达降低，敲低FOXA2可增加NCI-H292细胞增殖。FOXA2敲低减弱了姜黄素对细胞增殖的抑制作用。此外，姜黄素对STAT3通路的抑制增加了NCI-H292细胞中FOXA2的表达，而STAT3激活剂（IL-6）显著抑制姜黄素诱导的FOXA2表达。此外，姜黄素处理上调了STAT3活性的负调节因子SOCS1和SOCS3。因此，姜黄素通过调节STAT3信号通路增加FOXA2表达来抑制人NCI-H292细胞生长。
