Hou Chunlei, Zhu Xiaoli, Chang Xiangyun
Department of Pediatrics, Daqing Longnan Hospital, Daqing, Heilongjiang 163453, P.R. China.
Exp Ther Med. 2018 Mar;15(3):2773-2776. doi: 10.3892/etm.2018.5739. Epub 2018 Jan 11.
This study was designed to investigate the correlation of vitamin D receptor (VDR) gene polymorphism with bronchial asthma in children. Seventy patients admitted to Daqing Longnan Hospital and diagnosed as bronchial asthma for the first time from April 2015 to May 2017 were selected as observation group. Patients received routine treatment and intervention. Seventy healthy subjects admitted to hospital during the same period were enrolled as the control group. Vitamin D gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism assay; the levels of total serum immunoglobulin E (IgE) in the two groups were determined by electrochemiluminescence immunoassay; lung function levels in patients were measured using PowerCube (Germany) pulmonary function instrument before and after treatment, and the relationship between VDR gene polymorphism and lung function in children with bronchial asthma was analyzed. The difference in comparison of base frequency of rs1544410 locus of VDR gene had no statistical significance between the two groups (P>0.05). The locus CC frequency of rs7975232 of VDR gene in observation group was lower in the observation group than that in the control group (P<0.05); the locus AC and AA frequencies of rs7975232 of VDR gene were higher in the observation group than those in the control group (P<0.05). The level of vitamin D was lower in the observation group than that in the control group (P<0.05); the level of total serum IgE was higher in the observation group than that in the control group (P<0.05). The forced expiratory volume in 1 sec (FEV1), peak expiratory flow (PEF) and the ratio of FEV1 to forced vital capacity (FVC) in children with bronchial asthma in the observation group were higher after treatment than those before treatment (P<0.05). The correlation research displayed that VDR gene polymorphism was negatively correlated with lung function levels in children with bronchial asthma (P<0.05). The results showed that children with bronchial asthma are often accompanied by different degrees of changes in VDR gene polymorphism, which is negatively correlated with the severity of asthma, so vitamin D should be strengthened to ameliorate the prognosis of children.
本研究旨在探讨维生素D受体（VDR）基因多态性与儿童支气管哮喘的相关性。选取2015年4月至2017年5月在大庆龙南医院首次确诊为支气管哮喘的70例患儿作为观察组，给予常规治疗及干预。选取同期住院的70例健康儿童作为对照组。采用聚合酶链反应-限制性片段长度多态性分析法检测维生素D基因多态性；采用电化学发光免疫分析法测定两组血清总免疫球蛋白E（IgE）水平；治疗前后采用德国PowerCube肺功能仪测定患儿肺功能水平，并分析VDR基因多态性与儿童支气管哮喘肺功能的关系。两组VDR基因rs1544410位点碱基频率比较差异无统计学意义（P>0.05）。观察组VDR基因rs7975232位点CC基因型频率低于对照组（P<0.05）；观察组VDR基因rs7975232位点AC和AA基因型频率高于对照组（P<0.05）。观察组维生素D水平低于对照组（P<0.05）；观察组血清总IgE水平高于对照组（P<0.05）。观察组支气管哮喘患儿治疗后1秒用力呼气容积（FEV1）、呼气峰值流速（PEF）及FEV1与用力肺活量（FVC）比值均高于治疗前（P<0.05）。相关性研究显示，VDR基因多态性与支气管哮喘患儿肺功能水平呈负相关（P<0.05）。结果表明，支气管哮喘患儿常伴有不同程度的VDR基因多态性改变，且与哮喘严重程度呈负相关，故应加强补充维生素D以改善患儿预后。
