Kilic Mehmet, Ecin Sema, Taskin Erdal, Sen Askin, Kara Murat
Division of Allergy and Immunology, Department of Pediatrics, University of Firat, Elazig, Turkey.
Department of Pediatrics, Faculty of Medicine, University of Firat, Elazig, Turkey.
Pediatr Allergy Immunol Pulmonol. 2019 Jun 1;32(2):63-69. doi: 10.1089/ped.2018.0948. Epub 2019 Jun 17.
The association between vitamin D receptor (VDR) polymorphisms and the risk of asthma remains unclear. This study aimed to investigate the effect of VDR gene polymorphisms and VDR mRNA expression levels on respiratory function, nitric oxide levels in expiratory air, and serum vitamin D levels in children with asthma. The study included 80 healthy children (control group) and 100 asthmatic children (asthma group) between the age of 5 and 18 years. The VDR genotypes (I, I, and I) and VDR mRNA levels were determined in all groups. There was no statistically significant difference in vitamin D levels between the asthma group and the control group ( > 0.05). A significant association was found between both genotype (CC) of the I polymorphism [odds ratio (OR) = 0.2, 95% confidence interval (CI) (0.07-0.5), = 0.003] and genotype (CA) of I polymorphisms [OR = 0.2, 95% CI (0.07-0.8), = 0.02], and asthma risk. In addition, when single-nucleotide polymorphism allelic frequencies between asthma and control groups were compared there is no significant association ( > 0.05). When compared to control group, VDR mRNA expression in asthma group decreased in genotypes CC and CA of I and in genotypes TT and TC of I ( < 0.05). The results provide supporting evidence for an association between I and I polymorphisms and asthma susceptibility.
维生素D受体（VDR）基因多态性与哮喘风险之间的关联尚不清楚。本研究旨在探讨VDR基因多态性和VDR mRNA表达水平对哮喘患儿呼吸功能、呼出气一氧化氮水平及血清维生素D水平的影响。该研究纳入了80名5至18岁的健康儿童（对照组）和100名哮喘儿童（哮喘组）。测定了所有组的VDR基因型（I、I和I）和VDR mRNA水平。哮喘组和对照组之间的维生素D水平无统计学显著差异（P>0.05）。发现I多态性的基因型（CC）[比值比（OR）=0.2，95%置信区间（CI）（0.07-0.5），P=0.003]和I多态性的基因型（CA）[OR=0.2，95%CI（0.07-0.8），P=0.02]均与哮喘风险存在显著关联。此外，比较哮喘组和对照组之间的单核苷酸多态性等位基因频率时，无显著关联（P>0.05）。与对照组相比，哮喘组中I的基因型CC和CA以及I的基因型TT和TC的VDR mRNA表达降低（P<0.05）。研究结果为I和I多态性与哮喘易感性之间的关联提供了支持证据。
