Department of Biology, University Campus 2, University of Guilan, Rasht, Iran.
Department of Biology, Faculty of Science, University of Guilan, 5th Kilometer of Persian Gulf Highway, Rasht, Guilan Province, Iran 4199613776.
Biomed Res Int. 2021 Jun 1;2021:9981915. doi: 10.1155/2021/9981915. eCollection 2021.
Antibacterial activity of ethanolic and aqueous extracts of two medicinal plants including (EtOc, AqOc) and (EtAa, AqAa) as well as essential oil (EoAa) was investigated on multi-drug resistance (MDR) . Microdilution and agar well diffusion methods were used to determine the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC) as well as the inhibition zone. The phytconstituents of these products were analyzed using Reverse-phase High- performance liquid chromatography (RP-HPLC) and gas chromatography-mass spectrometry (GC-mass). The order of bacteriostatic and bacteriocide rate of the products can be shown as follows: EoAa>AqOc>EtAa = AqAa>EtOc, but the bactericidal effect of extracts is higher than of based on the MIC/MBC ratio and the order is as follows: EoAa>EtAa = AqAa>EtOc>AqOc. The most potent product, i.e. EoAa with a 56.7% inhibition of all isolates, has the potential to substitute 13 used antibiotics including oxacillin, amoxicillin, ampicillin, amoxicillin-clavulanic acid, tetracycline, streptomycin, ciprofloxacin, ceftriaxone, cefazolin, cefuroxime, cefotaxime, ceftazidime and cefixime (). Different terpenoids were detected and measured in EoAa and catechin flavonoids in extracts of both plants, quercetin in extracts of but it was only possible to detect chlorogenic acid polyphenol in AqAa. Due to the antibacterial activities of the studied products, more effective than some antibiotics and their edible consumption, these products can be suggested as an alternative to some antibiotics and food preservatives to fight against MDR
两种药用植物的乙醇和水提取物（EtOc、AqOc）和（EtAa、AqAa）以及精油（EoAa）的抗菌活性对多药耐药（MDR）进行了研究。采用微量稀释法和琼脂孔扩散法测定最小抑菌浓度（MIC）、最小杀菌浓度（MBC）和抑菌圈。采用反相高效液相色谱法（RP-HPLC）和气相色谱-质谱法（GC-MS）分析这些产品的植物成分。产品的抑菌和杀菌率顺序如下：EoAa>AqOc>EtAa=AqAa>EtOc，但根据 MIC/MBC 比值，提取物的杀菌效果高于，顺序如下：EoAa>EtAa=AqAa>EtOc>AqOc。最有效的产品，即对所有分离物抑制率为 56.7%的 EoAa，具有替代包括苯唑西林、阿莫西林、氨苄西林、阿莫西林-克拉维酸、四环素、链霉素、环丙沙星、头孢曲松、头孢唑林、头孢呋辛、头孢噻肟、头孢他啶和头孢克肟在内的 13 种抗生素的潜力（）。在 EoAa 中检测到并测量了不同的萜类化合物，在两种植物的提取物中检测到儿茶素类黄酮，在 的提取物中检测到槲皮素，但只在 AqAa 中检测到绿原酸多酚。由于研究产品的抗菌活性比一些抗生素更有效，并且可以食用，因此这些产品可以替代一些抗生素和食品防腐剂，用于对抗 MDR。
