Pharmaceutics Department, Faculty of Pharmacy, Damanhour University, Egypt.
Pharmaceutical Technology Department, National Research Centre, El-Bohooth Street, Cairo, Egypt.
Int J Pharm. 2018 Apr 25;541(1-2):37-47. doi: 10.1016/j.ijpharm.2018.01.054. Epub 2018 Feb 17.
In this study, Compritol ATO-based Resveratrol colloidal carriers (CCCs) were prepared and subjected to characterization and evaluation. In most formulae, the use of a binary-mixture of surfactants improved the physicochemical properties. CCC6 (containing P407/P188 as bi-surfactants) attained the highest drug loading, release efficiency during 24 h and occlusive effect for 48 h; in addition, it showed a uniform particle size distribution within the desired range. In-vivo studies were done based on the analysis of anti-oxidant markers [catalase (CAT), reduced glutathione (GSH) and superoxide dismutase (SOD)], anti-inflammatory markers [interleukin 6 (IL-6), interleukin 8 (IL-8) and rat Nuclear factor-kappa B (NF-κB)] and anti-wrinkling markers [matrix metalloproteinase (MMP-1) and Granulocyte-macrophage colony-stimulating factor (GM-CSF)], after UVB-irradiation. Results were significantly different when comparing the positive control and the negative control groups (p < 0.05). Rats pre-treated with CCC6 showed a great amelioration, and the level of the biochemical markers was significantly different compared to those of the positive control group and those pre-treated with the drug suspension (p < 0.05). Also, the high skin protective effect of CCC6 was proved by visual and histopathological examination of the rats' skin. Therefore, the current study proves the beneficial effects of the designed dermal Resveratrol-loaded colloidal system.
在这项研究中，制备了基于 Compritol ATO 的白藜芦醇胶束载体（CCCs），并对其进行了表征和评价。在大多数配方中，使用二元混合物表面活性剂可改善物理化学性质。 CCC6（含有 P407/P188 作为双表面活性剂）具有最高的载药量、24 小时内的释放效率和 48 小时的封闭效果；此外，它显示出所需范围内均匀的粒径分布。基于抗氧化标志物[过氧化氢酶（CAT）、还原型谷胱甘肽（GSH）和超氧化物歧化酶（SOD）]、抗炎标志物[白细胞介素 6（IL-6）、白细胞介素 8（IL-8）和核因子-κB（NF-κB）]和抗皱标志物[基质金属蛋白酶 1（MMP-1）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）]的分析，进行了体内研究，在进行 UVB 照射后。阳性对照组和阴性对照组的结果有显著差异（p<0.05）。与阳性对照组和药物混悬剂预处理组相比，用 CCC6 预处理的大鼠有明显改善，生化标志物水平有显著差异（p<0.05）。此外，通过对大鼠皮肤的视觉和组织病理学检查，证明了 CCC6 具有很高的皮肤保护作用。因此，本研究证明了设计的皮肤负载白藜芦醇胶束系统的有益效果。
