Zewail Mariam, Gaafar Passent M E, Youssef Nancy Abdel Hamid Abou, Ali Merhan E, Ragab Mai F, Kamal Miranda F, Noureldin Mohamed H, Abbas Haidy
Pharmaceutics Department, Faculty of Pharmacy, Damanhour University, Damanhour P.O. Box 22511, Egypt.
Department of Pharmaceutics, Division of Pharmaceutical Sciences, College of Pharmacy, Arab Academy for Science, Technology and Maritime Transport, Alexandria P.O. Box 1029, Egypt.
Pharmaceuticals (Basel). 2022 Dec 27;16(1):36. doi: 10.3390/ph16010036.
The recent interest in bioactive compounds from natural sources has led to the evolution of the skin care industry. Efforts to develop biologically active ingredients from natural sources have resulted in the emergence of enhanced skin care products. Spirulina (SPR), a nutritionally enriched cyanobacteria-type microalga, is rich in nutrients and phytochemicals. SPR possesses antioxidant, immunomodulatory, and anti-inflammatory activities. Spirulina-loaded bilosomes (SPR-BS), a novel antiaging drug delivery system, were designed for the first time by incorporation in a lecithin−bile salt-integrated system for bypassing skin delivery obstacles. The optimized BS had good entrapment efficiency, small particle size, optimal zeta potential, and sustained drug release pattern. Blank and SPR-loaded BS formulations were safe, with a primary irritancy index of <2 based on the Draize test. In vivo tests were conducted, and photoprotective antiaging effects were evaluated visually and biochemically by analyzing antioxidant, anti-inflammatory, and anti-wrinkling markers following ultraviolet (UV) B irradiation. Results of biochemical marker analysis and histopathological examination confirmed the superior antiaging effect of SPR-BS compared with SPR. Thus, SPR-loaded BS is a promising nanoplatform for SPR delivery, can be used for treating UV-induced skin damage, and offers maximum therapeutic outcomes.
近期对天然来源生物活性化合物的关注推动了护肤品行业的发展。从天然来源开发生物活性成分的努力催生了功效更强的护肤产品。螺旋藻（SPR）是一种营养丰富的蓝细菌型微藻，富含营养物质和植物化学物质。SPR具有抗氧化、免疫调节和抗炎活性。载有螺旋藻的双分子层脂质体（SPR-BS）是一种新型抗衰老药物递送系统，首次通过将其掺入卵磷脂-胆盐整合系统中设计而成，以绕过皮肤递送障碍。优化后的双分子层脂质体具有良好的包封率、小粒径、最佳的zeta电位和持续的药物释放模式。空白和载有SPR的双分子层脂质体制剂是安全的，根据Draize试验，其原发性刺激指数<2。进行了体内试验，并通过分析紫外线（UV）B照射后的抗氧化、抗炎和抗皱标志物，从视觉和生化方面评估了光保护抗衰老效果。生化标志物分析和组织病理学检查结果证实，与SPR相比，SPR-BS具有更优异的抗衰老效果。因此，载有SPR的双分子层脂质体是一种有前景的用于递送SPR的纳米平台，可用于治疗紫外线引起的皮肤损伤，并能实现最大的治疗效果。
