Mashima Izumi, Liao Yu-Chieh, Miyakawa Hiroshi, Theodorea Citra F, Thawboon Boonyanit, Thaweboon Sroisiri, Scannapieco Frank A, Nakazawa Futoshi
Japan Society for the Promotion of Science, 5-3-1, Kouji-machi, Chiyoda-ku, Tokyo 102-0083, Japan.
Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, 3435 Main Street, Foster Hall, Buffalo, NY 14214, USA.
Int J Syst Evol Microbiol. 2018 Apr;68(4):1101-1106. doi: 10.1099/ijsem.0.002632. Epub 2018 Feb 16.
A strain of a novel anaerobic, Gram-stain-negative coccus was isolated from the tongue biofilm of a Thai child. This strain was shown, at the phenotypic level and based on 16S rRNA gene sequencing, to be a member of the genus Veillonella. Comparative analysis of the 16S rRNA, dnaK and rpoB gene sequences indicated that phylogenetically the strain comprised a distinct novel branch within the genus Veillonella. The novel strain showed 99.8, 95.1 and 95.9 % similarity to partial 16S rRNA, dnaK and rpoB gene sequences, respectively, to the type strains of the two most closely related species, Veillonelladispar ATCC 17748 and Veillonellatobetsuensis ATCC BAA-2400. The novel strain could be discriminated from previously reported species of the genus Veillonella based on partial dnaK and rpoB gene sequencing and average nucleotide identity values. The major acid end-product produced by this strain was acetic acid under anaerobic conditions in trypticase-yeast extract-haemin with 1 % (w/v) glucose or fructose medium. Lactate was fermented to acetic acid and propionic acid. Based on these observations, this strain represents a novel species, for which the name Veillonella infantium sp. nov. is proposed. The type strain is T11011-4 (=JCM 31738=TSD-88).
从一名泰国儿童的舌生物膜中分离出一株新型厌氧革兰氏阴性球菌。在表型水平并基于16S rRNA基因测序显示，该菌株属于韦荣氏菌属。对16S rRNA、dnaK和rpoB基因序列的比较分析表明，从系统发育角度来看，该菌株在韦荣氏菌属内构成一个独特的新分支。该新菌株与两个亲缘关系最近的物种——差异韦荣氏菌ATCC 17748和户别韦荣氏菌ATCC BAA - 2400的模式菌株的部分16S rRNA、dnaK和rpoB基因序列的相似性分别为99.8%、95.1%和95.9%。基于部分dnaK和rpoB基因测序以及平均核苷酸同一性值，该新菌株可与韦荣氏菌属先前报道的物种区分开来。在含有1%（w/v）葡萄糖或果糖的胰蛋白酶 - 酵母提取物 - 血红素厌氧条件下，该菌株产生的主要酸性终产物是乙酸。乳酸发酵生成乙酸和丙酸。基于这些观察结果，该菌株代表一个新物种，为此提出名为婴儿韦荣氏菌（Veillonella infantium sp. nov.）的名称。模式菌株为T11011 - 4（=JCM 31738 = TSD - 88）。
