State Key Laboratory for Agrobiotechnology, China Agricultural University, Beijing, 100193, People's Republic of China.
Antonie Van Leeuwenhoek. 2022 Jan;115(1):7-18. doi: 10.1007/s10482-021-01677-6. Epub 2021 Oct 31.
Two strains HN-1 and 39 were isolated from rhizospheres of different plants grown in different regions of PR China. The two strains exhibited high nitrogenase activities and possessed almost identical 16S rRNA gene sequences. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between the two strains were 99.9 and 99.8%, respectively, suggesting that they belong to one species. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strains HN-1 and 39 are the members of the genus Paenibacillus and both strains exhibited 99.5% similarity to Paenibacillus stellifer DSM 14472 and the both strains represented a separate lineage from all other Paenibacillus species. However, the ANI of type strain HN-1 with P. stellifer DSM 14472 was 90.69, which was below the recommended threshold value (< 95-96% ANI). The dDDH showed 42.1% relatedness between strain HN-1 and P. stellifer DSM 14472, which was lower than the recommended threshold value (dDDH < 70%). The strain HN-1 contain anteiso-C as major fatty acids and MK-7 as predominant isoprenoid quinone. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four aminophospholipids and an unidentified glycolipid. Unlike the most closely related P. stellifer DSM 14472, strain HN-1 or 39 was positive for catalase reaction. Distinct phenotypic and genomic characterisations from previously described taxa support the classification of strains HN-1 or 39 as representatives of a novel species of the genus Paenibacillus, for which the name Paenibacillus sinensis is proposed, with type strains HN-1 (=CGMCC 1.18902, JCM 34,620), and reference strain 39 (=CGMCC 1.18879, JCM 34,616), respectively.
两株 HN-1 和 39 菌株从中国不同地区不同植物的根际土壤中分离得到。这两株菌具有较高的固氮酶活性，且 16S rRNA 基因序列几乎完全相同。两株菌的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）值分别为 99.9%和 99.8%，表明它们属于同一个种。基于 16S rRNA 基因序列的系统发育分析表明，菌株 HN-1 和 39 是类芽孢杆菌属的成员，两株菌与类芽孢杆菌 stellifer DSM 14472 的相似度均为 99.5%，且两株菌均与其他所有类芽孢杆菌物种形成单独的分支。然而，HN-1 株与 P. stellifer DSM 14472 的 ANI 为 90.69，低于推荐的阈值（<95-96% ANI）。dDDH 显示菌株 HN-1 与 P. stellifer DSM 14472 之间的相关性为 42.1%，低于推荐的阈值（dDDH<70%）。菌株 HN-1 含有 anteiso-C 作为主要脂肪酸和 MK-7 作为主要异戊烯醌。主要极性脂是双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、四种氨基磷脂和一种未知的糖脂。与最密切相关的 P. stellifer DSM 14472 不同，菌株 HN-1 或 39 对过氧化氢酶反应呈阳性。与先前描述的分类群的明显表型和基因组特征支持菌株 HN-1 或 39 被分类为类芽孢杆菌属的一个新种的代表，建议将其命名为中华类芽孢杆菌，其模式菌株为 HN-1（=CGMCC 1.18902，JCM 34,620）和参考菌株 39（=CGMCC 1.18879，JCM 34,616）。
