Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, Germany.
Max Planck Institute for Marine Microbiology, Bremen, Germany.
Nat Chem Biol. 2018 Apr;14(4):342-344. doi: 10.1038/s41589-018-0005-8. Epub 2018 Feb 19.
Sugar O-methylation shields algal polysaccharides against microbial hydrolytic enzymes. Here, we describe cytochrome P450 monooxygenases from marine bacteria that, together with appropriate redox-partner proteins, catalyze the oxidative demethylation of 6-O-methyl-D-galactose, which is an abundant monosaccharide of the algal polysaccharides agarose and porphyran. This previously unknown biological function extends the group of carbohydrate-active enzymes to include the class of cytochrome P450 monooxygenases.
糖-O-甲基化能保护藻类多糖免受微生物水解酶的作用。在这里，我们描述了来自海洋细菌的细胞色素 P450 单加氧酶，这些酶与合适的氧化还原伴侣蛋白一起，催化 6-O-甲基-D-半乳糖的氧化脱甲基反应，6-O-甲基-D-半乳糖是琼脂糖和褐藻胶等藻类多糖的一种丰富单糖。这一先前未知的生物学功能将糖基活性酶的类别扩展到包括细胞色素 P450 单加氧酶。
