Department of Biotechnology & Enzyme Catalysis, Institute of Biochemistry, University of Greifswald, Greifswald, 17487, Germany.
Laboratory of Integrative Biology of Marine Models (LBI2 M), Station Biologique de Roscoff (SBR), Sorbonne Université, CNRS, 29688, Roscoff, Bretagne, France.
Chembiochem. 2022 Jul 19;23(14):e202200269. doi: 10.1002/cbic.202200269. Epub 2022 May 30.
Formaldehyde is a toxic metabolite that is formed in large quantities during bacterial utilization of the methoxy sugar 6-O-methyl-d-galactose, an abundant monosaccharide in the red algal polysaccharide porphyran. Marine bacteria capable of metabolizing porphyran must therefore possess suitable detoxification systems for formaldehyde. We demonstrate here that detoxification of formaldehyde in the marine Flavobacterium Zobellia galactanivorans proceeds via the ribulose monophosphate pathway. Simultaneously, we show that the genes encoding the key enzymes of this pathway are important for maintaining high formaldehyde resistance. Additionally, these genes are upregulated in the presence of porphyran, allowing us to connect porphyran degradation to the detoxification of formed formaldehyde.
甲醛是一种有毒的代谢物，在细菌利用丰富的红藻多糖褐藻糖胶中的甲氧基糖 6-O-甲基-d-半乳糖时会大量形成。因此，能够代谢褐藻糖胶的海洋细菌必须拥有合适的甲醛解毒系统。我们在这里证明，海洋黄杆菌 Zobellia galactanivorans 中甲醛的解毒是通过核酮糖单磷酸途径进行的。同时，我们表明，该途径关键酶的编码基因对于维持高甲醛抗性很重要。此外，这些基因在褐藻聚糖存在的情况下被上调，这使我们能够将褐藻聚糖的降解与形成的甲醛的解毒联系起来。
