Rodríguez Alicia, Trigo Marcos, Aubourg Santiago P, Medina Isabel
Department of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, C/Santos Dumont 964, Santiago 8380000, Chile.
Department of Food Technology, Marine Research Institute (CSIC), c/E. Cabello 6., 36208 Vigo, Spain.
Foods. 2023 Jan 21;12(3):504. doi: 10.3390/foods12030504.
The extraction of total lipids and tocopherol compounds from Patagonian squid () by-products (viscera, heads, skin, etc.), resulting from squid mantel commercialisation, was studied. An optimisation simplex-lattice design by employing low-toxicity solvents (ethanol, acetone, and ethyl acetate) was carried out taking into account their relative concentrations. The variance analysis of data showed that the quadratic model was statistically significant ( < 0.05); empirical coded equations were obtained as a function of the low-toxicity solvent ratios. The optimised lipid extraction was obtained by employing the 0.642/0.318/0.040 (ethanol/acetone/ethyl acetate) solvent ratio, respectively, leading to an 84% recovery of the total lipids extracted by the traditional procedure. In all extracting systems tested, the presence of α-, γ-, and δ-tocopherol compounds was detected, α-tocopherol being the most abundant. For α-, γ-, and δ-tocopherol compounds, the optimisation process showed that acetone extraction led to the highest concentrations in the lipid extract obtained (2736.5, 36.8, and 2.8 mg·kg lipids, respectively). Taking into account the recovery yield on a by-product basis, the values obtained for the three tocopherols were included in the 88.0-97.7%, 80.0-95.0%, and 25-75% ranges, respectively, when compared to the traditional extraction. This study provides a novel and valuable possibility for α-tocopherol extraction from marine by-products.
研究了从鱿鱼商业加工产生的巴塔哥尼亚鱿鱼（）副产品（内脏、头部、皮肤等）中提取总脂质和生育酚化合物的方法。考虑到低毒性溶剂（乙醇、丙酮和乙酸乙酯）的相对浓度，采用优化的单纯形格子设计进行实验。数据的方差分析表明二次模型具有统计学意义（<0.05）；根据低毒性溶剂比例得到了经验编码方程。通过采用0.642/0.318/0.040（乙醇/丙酮/乙酸乙酯）的溶剂比例分别获得了优化的脂质提取效果，与传统方法相比，总脂质回收率达到84%。在所有测试的提取系统中，均检测到α-、γ-和δ-生育酚化合物的存在，其中α-生育酚含量最高。对于α-、γ-和δ-生育酚化合物，优化过程表明丙酮提取法可使脂质提取物中这些化合物的浓度达到最高（分别为2736.5、36.8和2.8mg·kg脂质）。从副产品的回收率来看，与传统提取方法相比，三种生育酚的回收率分别在88.0 - 97.7%、80.0 - 95.0%和25 - 75%的范围内。本研究为从海洋副产品中提取α-生育酚提供了一种新颖且有价值的可能性。
