Méndez Lucía, Rodríguez Alicia, Aubourg Santiago P, Medina Isabel
Department of Food Technology, Marine Research Institute (CSIC), 36208 Vigo, Spain.
Department of Food Science and Chemical Technology, Faculty of Chemical and Pharmaceutical Sciences, University of Chile, Santiago 8380494, Chile.
Foods. 2023 Sep 30;12(19):3631. doi: 10.3390/foods12193631.
This study focused on the recovery of valuable lipid compounds from octopus () by-products. Extraction conditions of total lipids (TLs), phospholipids (PLs), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) were optimized by employing a Simplex-Lattice design; for it, different relative concentrations of three low-toxicity solvents (ethanol, acetone, and ethyl acetate) were considered. The optimization process was also addressed in reference to fatty acid (FA) ratios (total polyunsaturated FAs/total saturated FAs and total ω3 FAs/total ω6 FAs). The variance analysis of multiple regression data demonstrated that the quadratic model was significant ( < 0.05) for TL, PL, and DHA values and the ω3/ω6 ratio. As a result, the following optimized values were obtained: 113.8 g·kg dry by-products (TLs), 217.3 g·kg lipids (PLs), 22.55 g·100 g total FAs (DHA), and 3.70 (ω3/ω6 ratio). According to the model developed, optimized values were shown to correspond to the following relative solvent concentrations (ethanol/acetone/ethyl acetate): 0.46/0.00/0.54, 0.93/0.07/0.00, 0.83/0.17/0.00, and 0.64/0.00/0.36, respectively. Comparison to yields obtained by the conventional chloroform/methanol method was carried out. A novel strategy based on the employment of low-toxicity solvents is proposed for the extraction of valuable lipid constituents from octopus waste. A different solvent ratio would be necessary according to the lipid compound concerned.
本研究聚焦于从章鱼副产物中回收有价值的脂质化合物。采用单纯形格子设计优化了总脂质（TLs）、磷脂（PLs）、二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）的提取条件；为此，考虑了三种低毒溶剂（乙醇、丙酮和乙酸乙酯）的不同相对浓度。优化过程还参考了脂肪酸（FA）比率（总多不饱和脂肪酸/总饱和脂肪酸以及总ω3脂肪酸/总ω6脂肪酸）。多元回归数据的方差分析表明，二次模型对TL、PL、DHA值以及ω3/ω6比率具有显著性（<0.05）。结果，获得了以下优化值：113.8 g·kg干副产物（TLs）、217.3 g·kg脂质（PLs）、22.55 g·100 g总脂肪酸（DHA）以及3.70（ω3/ω6比率）。根据所建立的模型，优化值分别对应以下相对溶剂浓度（乙醇/丙酮/乙酸乙酯）：0.46/0.00/0.54、0.93/0.07/0.00、0.83/0.17/0.00以及0.64/0.00/0.36。与传统氯仿/甲醇法获得的产率进行了比较。提出了一种基于使用低毒溶剂的新策略，用于从章鱼废弃物中提取有价值的脂质成分。根据所涉及的脂质化合物，需要不同的溶剂比例。
