Rocchetti Gabriele, Pellizzoni Marco, Montesano Domenico, Lucini Luigi
Department of Animal Science, Food and Nutrition, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, Italy.
Department for Sustainable Food Process, Università Cattolica del Sacro Cuore, via Emilia Parmense 84, 29122 Piacenza, Italy.
Foods. 2018 Feb 18;7(2):24. doi: 10.3390/foods7020024.
Natural by-products, especially phenolic compounds, are in great demand by the nutra-pharmaceutical and biomedical industries. An analytical study was performed to investigate, for the first time, the presence of antioxidant constituents and the corresponding in vitro antioxidant activity in the extract of cladodes from Ficodindia di San Cono () protected designation of origin (PDO). The cladode extracts were analysed for target determination of selected constituents, i.e. β-polysaccharides and total phenolic content. Moreover, the antioxidant activity of hydro-alcoholic extracts was assessed by means of two different methods: α, α-diphenyl-β-picrylhydrazyl (DPPH) free radical scavenging method and ferric reducing antioxidant power (FRAP) assay. An untargeted UHPLC-ESI-QTOF-MS profiling approach was used to depict the phenolic profile of hydro-alcoholic cladode extracts. Interestingly, over 2 g/kg of polyphenols were detected in this matrix, and these compounds were mainly responsible for the antioxidant properties, as shown by the strong correlation between phenolic classes and antioxidant scores. Finally, this study provides basic information on the presence of bioactive compounds and in vitro antioxidant activities in cladode extracts from cactus that might recommend their novel applications at the industrial level in the field of nutraceutical products.
天然副产品，尤其是酚类化合物，在营养制药和生物医学行业中需求量很大。首次进行了一项分析研究，以调查受保护的原产地名称（PDO）菲科迪迪亚·迪·圣科诺仙人掌茎提取物中抗氧化成分的存在情况及其相应的体外抗氧化活性。对仙人掌茎提取物进行分析，以确定选定成分，即β-多糖和总酚含量。此外，通过两种不同方法评估水醇提取物的抗氧化活性：α，α-二苯基-β-苦基肼（DPPH）自由基清除法和铁还原抗氧化能力（FRAP）测定法。采用非靶向超高效液相色谱-电喷雾电离-四极杆飞行时间质谱（UHPLC-ESI-QTOF-MS）分析方法来描绘水醇仙人掌茎提取物的酚类特征。有趣的是，在该基质中检测到超过2 g/kg的多酚，并且这些化合物是抗氧化特性的主要原因，酚类类别与抗氧化评分之间的强相关性表明了这一点。最后，本研究提供了关于仙人掌茎提取物中生物活性化合物的存在情况及其体外抗氧化活性的基本信息，这可能会推荐它们在营养保健品领域的工业层面上的新应用。
