Lima Serena, Webb Conner L, Deery Evelyne, Robinson Colin, Zedler Julie A Z
Industrial Biotechnology Centre, School of Biosciences, University of Kent, Giles Lane, Canterbury, Kent CT2 7 NK, UK.
Dipartimento dell'Innovazione Industriale e Digitale, Università degli Studi di Palermo, Viale delle Scienze Ed. 6, 90128 Palermo, Italy.
Biology (Basel). 2018 Feb 19;7(1):19. doi: 10.3390/biology7010019.
Dietary supplements and functional foods are becoming increasingly popular complements to regular diets. A recurring ingredient is the essential cofactor vitamin B (B). Microalgae are making their way into the dietary supplement and functional food market but do not produce B, and their B content is very variable. In this study, the suitability of using the human B-binding protein intrinsic factor (IF) to enrich bioavailable B using microalgae was tested. The IF protein was successfully expressed from the nuclear genome of the model microalga and the addition of an N-terminal ARS2 signal peptide resulted in efficient IF secretion to the medium. Co-abundance of B and the secreted IF suggests the algal produced IF protein is functional and B-binding. Utilizing IF expression could be an efficient tool to generate B-enriched microalgae in a controlled manner that is suitable for vegetarians and, potentially, more bioavailable for humans.
膳食补充剂和功能性食品正日益成为常规饮食的流行补充品。一种常见成分是必需辅因子维生素B（B族维生素）。微藻正进入膳食补充剂和功能性食品市场，但它们不产生B族维生素，且其B族维生素含量变化很大。在本研究中，测试了使用人类B族维生素结合蛋白内因子（IF）利用微藻富集生物可利用B族维生素的适用性。IF蛋白成功地从模式微藻的核基因组中表达出来，添加N端ARS2信号肽导致IF有效分泌到培养基中。B族维生素与分泌的IF共同丰富表明藻类产生的IF蛋白具有功能且能结合B族维生素。利用IF表达可能是一种有效的工具，可用于以可控方式生产富含B族维生素的微藻，这种微藻适合素食者，并且对人类可能具有更高的生物可利用性。
