Stojkovska Jasmina, Djurdjevic Zeljka, Jancic Ivan, Bufan Biljana, Milenkovic Marina, Jankovic Radmila, Miskovic-Stankovic Vesna, Obradovic Bojana
1 Faculty of Technology and Metallurgy, University of Belgrade, Belgrade, Serbia.
2 Faculty of Pharmacy, University of Belgrade, Belgrade, Serbia.
J Biomater Appl. 2018 Apr;32(9):1197-1211. doi: 10.1177/0885328218759564. Epub 2018 Feb 20.
In the present study, possibilities for using novel nanocomposites based on alginate and silver nanoparticles for wound treatment were investigated in a second-degree thermal burn model in Wistar rats. Silver nanoparticles (AgNPs) were electrochemically synthesized in alginate solutions that were further utilized to obtain the Ag/alginate solution and microfibers for subsequent in vivo studies. Daily applications of the Ag/alginate colloid solution, containing AgNPs, alginate and ascorbic acid (G3), wet Ag/alginate microfibers containing AgNPs (G5) and dry Ag/alginate microfibers containing AgNPs (G6) were compared to treatments with a commercial cream containing silver sulfadiazine (G2) and a commercial Ca-alginate wound dressing containing silver ions (G4), as well as to the untreated controls (G1). Results of the in vivo study have shown faster healing in treated wounds, which completely healed on day 19 (G4, G5 and G6) and 21 (G2 and G3) after the thermal injury, while the period for complete reepitelization of untreated wounds (G1) was 25 days. The macroscopic analysis has shown that scabs fell off between day 10 and 12 after the thermal injury induction in treated groups, whereas between day 15 and 16 in the control group. These macroscopic findings were supported by the results of histopathological analyses, which have shown enhanced granulation and reepithelization, reduced inflammation and improved organization of the extracellular matrix in treated groups without adverse effects. Among the treated groups, dressings based on Ca-alginate (G4-G6) induced enhanced healing as compared to the other two groups (G2, G3), which could be attributed to additional stimuli of released Ca. The obtained results indicated potentials of novel nanocomposites based on alginate and AgNPs for therapeutic applications in wound treatments.
在本研究中，我们在Wistar大鼠的二度热烧伤模型中研究了基于藻酸盐和银纳米颗粒的新型纳米复合材料用于伤口治疗的可能性。银纳米颗粒（AgNPs）在藻酸盐溶液中通过电化学合成，该溶液进一步用于获得Ag/藻酸盐溶液和微纤维，用于后续的体内研究。将含有AgNPs、藻酸盐和抗坏血酸的Ag/藻酸盐胶体溶液（G3）、含有AgNPs的湿Ag/藻酸盐微纤维（G5）和含有AgNPs的干Ag/藻酸盐微纤维（G6）的每日应用与用含有磺胺嘧啶银的商业乳膏（G2）和含有银离子的商业Ca-藻酸盐伤口敷料（G4）进行的治疗进行比较，并与未治疗的对照组（G1）进行比较。体内研究结果表明，治疗后的伤口愈合更快，热损伤后第19天（G4、G5和G6）和第21天（G2和G3）完全愈合，而未治疗伤口（G1）完全重新上皮化的时间为25天。宏观分析表明，治疗组在热损伤诱导后第10至12天结痂脱落，而对照组在第15至16天结痂脱落。这些宏观发现得到了组织病理学分析结果的支持，该分析表明治疗组的肉芽组织增生和重新上皮化增强，炎症减轻，细胞外基质组织改善，且无不良反应。在治疗组中，与其他两组（G2、G3）相比，基于Ca-藻酸盐的敷料（G4-G6）促进了愈合，这可能归因于释放的Ca的额外刺激。所获得的结果表明基于藻酸盐和AgNPs的新型纳米复合材料在伤口治疗中的治疗应用潜力。
