Stanisic Danijela, Fregonesi Natália L, Barros Caio H N, Pontes João G M, Fulaz Stephanie, Menezes Ulisses J, Nicoleti Jorge L, Castro Thiago L P, Seyffert Núbia, Azevedo Vasco, Durán Nelson, Portela Ricardo W, Tasic Ljubica
Laboratório de Química Biológica, Departamento de Química Orgânica, Instituto de Química, Universidade Estadual de Campinas Campinas SP Brazil
Laboratório de Imunologia e Biologia Molecular, Instituto de Ciências da Saúde, Universidade Federal da Bahia Salvador BA Brazil.
RSC Adv. 2018 Dec 5;8(71):40778-40786. doi: 10.1039/c8ra08218a. eCollection 2018 Dec 4.
Caseous lymphadenitis (CL), caused by a pathogen of the second class of biosafety - , is a chronic and severe infectious disease that affects small ruminants and requires long, ineffective treatment which generally leads to animal sacrifice so as to stop the disease spreading. The infected animals suffer the excision of affected superficial lymph nodes and post-surgical treatment with iodine (10% solution in ethanol) and, sometimes, prolonged antibiotic use, but only if the sick animals are of great importance to breeding. Herein, we propose a cheap and easy to apply treatment of CL with excellent results using biogenic silver nanoparticles (AgNP) based technology. AgNP antibacterial properties were investigated against cells and on small ruminants with CL. Treatment of surgical wounds resulting from the excision of superficial CL lesions with a AgNP-based cream was compared to the standard post-surgical treatment method by iodine. Also, the effects of AgNP-based cream treatment were evaluated and compared with the effects of the iodine CL treatment by serum NMR-based metabolomics. Serum samples were collected from 29 animals, 9 sheep and 20 goats, during the treatments and analyzed. All animals showed stable serum metabolomes when iodine or AgNP-based cream effects were compared. The AgNP-based cream treatment showed excellent results, especially in accelerating the healing of wounds, which occurred two to three times faster in comparison with the iodine treatment. AgNP-based cream treatment also prevented CL reappearance and did not cause any side effects on animals. This is the first report on very effective post-surgical treatment of superficial CL in small ruminants based on biogenic silver nanoparticles, which might open up the possibility for a safe veterinary application of AgNP-based cream.
干酪性淋巴结炎（CL）由生物安全二级病原体引起，是一种影响小型反刍动物的慢性严重传染病，需要长期进行无效治疗，通常会导致动物被宰杀以防止疾病传播。受感染动物要切除受影响的浅表淋巴结，并在术后用碘（乙醇中10%的溶液）治疗，有时还需要长期使用抗生素，但前提是患病动物对繁殖非常重要。在此，我们提出一种基于生物源银纳米颗粒（AgNP）技术的廉价且易于应用的CL治疗方法，效果极佳。研究了AgNP对细胞和患有CL的小型反刍动物的抗菌性能。将基于AgNP的乳膏用于治疗浅表CL病变切除术后的手术伤口，并与碘的标准术后治疗方法进行比较。此外，通过基于血清核磁共振的代谢组学评估基于AgNP的乳膏治疗效果，并与碘治疗CL的效果进行比较。在治疗期间从29只动物（9只绵羊和20只山羊）采集血清样本并进行分析。在比较碘或基于AgNP的乳膏的效果时，所有动物的血清代谢组均显示稳定。基于AgNP的乳膏治疗效果极佳，尤其是在加速伤口愈合方面，与碘治疗相比，伤口愈合速度快两到三倍。基于AgNP的乳膏治疗还可防止CL复发，且对动物未造成任何副作用。这是第一份关于基于生物源银纳米颗粒对小型反刍动物浅表CL进行非常有效的术后治疗的报告，这可能为基于AgNP的乳膏在兽医领域的安全应用开辟可能性。
