Neurobiology Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India.
Center of Biomedical Research, Sanjay Gandhi Postgraduate Institute of Medical Sciences, Lucknow, 226014, India.
Mol Cell Biochem. 2018 Nov;448(1-2):107-124. doi: 10.1007/s11010-018-3318-8. Epub 2018 Feb 21.
Collagen-induced arthritis (CIA) animal model is associated with systemic manifestations, including alteration of lipid metabolism. In the present study, one possible pathway of altered lipid metabolism is proposed. Specimens of joint tissue and plasma were collected from the CIA and control rats, and quantitative analysis of lipid components was performed by nuclear magnetic resonance (NMR) spectroscopy technique. Correlation analysis was performed between the level of lipid components and antioxidant enzymes, lactate dehydrogenase (LDH), lipid peroxidation (LP), and cytokines in joint tissue and plasma. Differentiation between the CIA and control rats was established on the basis of the quantity of lipid components in the joint tissue and plasma. Positive correlation was observed for all the enzymes vs. lipid components as well as LP vs. lipid components in plasma and joint tissue. Positive correlation was observed for enzymes in plasma and joint tissue. A negative correlation was observed in between the plasma and joint tissue with the level of lipid components. Cytokine levels were also correlated with the level of lipid components and ratios of saturated fatty acids/unsaturated fatty acids in plasma and joint tissue. Inflammatory disease activity in CIA rats with synovitis brought about a significant change in lipid metabolism. Taken together, the results of our study are delineating a possible pathway of altered lipid metabolism in the CIA rat model, thereby contributing further to an understanding of the pathophysiology of rheumatoid arthritis (RA).
胶原诱导性关节炎（CIA）动物模型与全身表现有关，包括脂质代谢的改变。在本研究中，提出了一种可能的脂质代谢改变途径。从 CIA 和对照大鼠的关节组织和血浆中采集标本，并通过核磁共振（NMR）光谱技术对脂质成分进行定量分析。对关节组织和血浆中脂质成分与抗氧化酶、乳酸脱氢酶（LDH）、脂质过氧化（LP）和细胞因子的水平进行相关性分析。基于关节组织和血浆中脂质成分的量来区分 CIA 和对照大鼠。所有酶与脂质成分以及 LP 与血浆和关节组织中的脂质成分均呈正相关。观察到酶在血浆和关节组织中的正相关。观察到血浆和关节组织中脂质成分的负相关。细胞因子水平与脂质成分以及血浆和关节组织中饱和脂肪酸/不饱和脂肪酸的比值也相关。在 CIA 大鼠中，滑膜炎引起的炎症性疾病活动导致脂质代谢发生显著变化。总之，我们的研究结果描绘了 CIA 大鼠模型中改变的脂质代谢的可能途径，从而进一步有助于理解类风湿关节炎（RA）的病理生理学。
