Departamento de Microbiología y Parasitología, CIBUS-Facultad de Biología, Universidade de Santiago de Compostela, 15782, Santiago de Compostela, Spain.
Plataforma Solar de Almería, CIEMAT, Tabernas, Spain.
Food Environ Virol. 2018 Jun;10(2):159-166. doi: 10.1007/s12560-018-9339-3. Epub 2018 Feb 21.
This study evaluates and compares the effectiveness of solar photo-Fenton systems for the inactivation of hepatitis A virus (HAV) in water. The effect of solar irradiance, dark- Fenton reaction and three different reactant concentrations (2.5/5, 5/10 and 10/20 mg/L of Fe/HO) on the photo-Fenton process were tested in glass bottle reactors (200 mL) during 6 h under natural sunlight. Disinfection kinetics were determined both by RT-qPCR and infectivity assays. Mean water temperatures ranged from 25 to 27.3 °C, with a maximum local noon UV irradiances of 22.36 W/m. Photo-Fenton systems yielded increased viral reduction rates in comparison with the isolated effect under the Fenton reaction in darkness (negligible viral reduction) or the solar radiation (0.25 Log of RNA reduction). With the highest concentration employed (10-20 mg/L Fe-HO), an average RNA reduction rate of ~ 1.8 Log (initial concentration of 10 pfu/mL) and a reduction of 80% in the infectivity capacity were reached. Results showed a strong synergistic effect between Fe/HO and sunlight, demonstrating that significant disinfection rates of HAV under photo-Fenton systems may occur with relatively higher efficiency at middle environmental temperatures and without the need for an energy-intensive light source.
本研究评估和比较了太阳能光芬顿系统对水中甲型肝炎病毒（HAV）灭活的效果。在自然阳光下，通过玻璃瓶反应器（200 毫升）在 6 小时内测试了太阳辐照度、暗芬顿反应和三种不同反应物浓度（2.5/5、5/10 和 10/20 mg/L 的 Fe/HO）对光芬顿过程的影响。通过 RT-qPCR 和感染性测定确定了消毒动力学。平均水温范围为 25 至 27.3°C，最大局部正午紫外线辐照度为 22.36 W/m。与黑暗中芬顿反应（病毒减少可忽略不计）或太阳辐射（RNA 减少 0.25 对数）的单独作用相比，光芬顿系统产生了更高的病毒减少率。在使用最高浓度（10-20 mg/L Fe-HO）时，达到了约 1.8 Log（初始浓度为 10 pfu/mL）的 RNA 减少率和 80%的感染力降低。结果表明，Fe/HO 和阳光之间存在强烈的协同作用，表明在中环境温度下，通过光芬顿系统对 HAV 进行高效消毒可能会发生，而不需要能源密集型光源。
