Zhao Chun Yuan, Wang Wei, Yao Hong Chao, Wang Xin
Int Arch Allergy Immunol. 2018;175(4):209-219. doi: 10.1159/000486857. Epub 2018 Feb 22.
Suppressor of cytokine signaling 1 (SOCS1) and SOCS3 play important roles in T helper cell differentiation, which is involved with the pathologic mechanisms of allergic rhinitis (AR). The aim of this study was to evaluate the expression of SOCS1 and SOCS3 in AR and find their regulatory microRNAs (miRNAs) to provide a basis for the treatment of AR.
The expression of SOCS1 and SOCS3 were analyzed by real-time PCR, immunohistochemistry, and Western blot. The correlative regulatory miRNAs were detected by real-time PCR. Luciferase assays and AR mouse model experiments were applied to identify correlative miRNAs that target SOCS3.
SOCS1 and SOCS3 mRNA were upregulated in the nasal mucosa and peripheral blood mononuclear cells of AR compared with controls. The expression of SOCS3 protein was significantly increased in the nasal mucosa of AR. The immunohistochemical staining results showed that SOCS3 was similarly localized in the superficial epithelium, submucosal glands, and vascular endothelium in the nasal mucosa of AR subjects and controls. However, SOCS3 protein was especially localized in the inflammatory cells, such as eosinophils, monocytes, and lymphocytes.
SOCS3 was targeted by miR30a- 5p in AR. Further study should be performed to identify the regulatory effect of miR30a-5p in AR, which may provide insights into a new therapeutic strategy.
细胞因子信号转导抑制因子1（SOCS1）和SOCS3在辅助性T细胞分化中发挥重要作用，而辅助性T细胞分化与变应性鼻炎（AR）的病理机制有关。本研究旨在评估SOCS1和SOCS3在AR中的表达，并找到它们的调控微小RNA（miRNA），为AR的治疗提供依据。
采用实时荧光定量PCR、免疫组织化学和蛋白质印迹法分析SOCS1和SOCS3的表达。通过实时荧光定量PCR检测相关调控miRNA。应用荧光素酶报告基因检测和AR小鼠模型实验来鉴定靶向SOCS3的相关miRNA。
与对照组相比，AR患者鼻黏膜和外周血单个核细胞中SOCS1和SOCS3 mRNA表达上调。AR患者鼻黏膜中SOCS3蛋白表达显著增加。免疫组织化学染色结果显示，AR患者和对照组鼻黏膜中SOCS3在浅表上皮、黏膜下腺和血管内皮中的定位相似。然而，SOCS3蛋白尤其定位于嗜酸性粒细胞、单核细胞和淋巴细胞等炎症细胞中。
在AR中，miR30a-5p靶向SOCS3。应进一步研究以确定miR30a-5p在AR中的调控作用，这可能为新的治疗策略提供思路。
