Widmer Giovanni, Ferrer Lluís, Favrot Claude, Paps Judy, Huynh Kevin, Olivry Thierry
Department of Infectious Diseases and Global Health, Cummings School of Veterinary Medicine at Tufts University, 200 Westboro Road, North Grafton, MA, USA.
Department of Clinical Sciences, Cummings School of Veterinary Medicine at Tufts University, 200 Westboro Road, North Grafton, MA, USA.
BMC Vet Res. 2018 Feb 23;14(1):51. doi: 10.1186/s12917-018-1370-y.
As prednisone and ciclosporin can have immunosuppressive effects and have been considered potential predisposing factors for skin infections, we investigated the impact of these drugs on the diversity of the cutaneous microbiota, the abundance of Malassezia and infection with Papillomaviruses.
Six atopic, asymptomatic Maltese-beagle dogs were treated with ciclosporin for one month and then with prednisone for another month, with a one-month wash-out between treatments. The dogs were sampled on the abdomen and pinna before and after each treatment using a swab. Samples for Papillomavirus detection were obtained with cytobrush sticks. The bacterial microbiota was characterized using 16S amplicon high-throughput sequencing. Malassezia populations were quantified with nested real-time PCR targeting the ribosomal internal transcribed spacer 1. The diversity and composition of cutaneous microbiota was not impacted in a detectable manner by any of the treatments. As observed for the bacterial microbiota, Malassezia populations were not affected by treatment. Three dogs were positive for Papillomavirus at more than one timepoint, but an association with treatment was not apparent.
Ciclosporin and prednisone at doses used for the treatment of atopic dermatitis do not impact the canine cutaneous microbiota in a detectable manner.
由于泼尼松和环孢素具有免疫抑制作用，被认为是皮肤感染的潜在诱发因素，我们研究了这些药物对皮肤微生物群多样性、马拉色菌丰度和乳头瘤病毒感染的影响。
6只患有特应性皮炎、无症状的马尔济斯-比格犬先接受1个月的环孢素治疗，然后再接受1个月的泼尼松治疗，两次治疗之间有1个月的洗脱期。每次治疗前后，用拭子在犬的腹部和耳廓取样。用细胞刷棒获取用于检测乳头瘤病毒的样本。使用16S扩增子高通量测序对细菌微生物群进行表征。采用靶向核糖体内部转录间隔区1的巢式实时PCR对马拉色菌种群进行定量。任何一种治疗方法均未对皮肤微生物群的多样性和组成产生可检测到的影响。与细菌微生物群的情况一样，马拉色菌种群也未受治疗影响。3只犬在多个时间点乳头瘤病毒检测呈阳性，但与治疗的关联并不明显。
用于治疗特应性皮炎的剂量的环孢素和泼尼松不会对犬皮肤微生物群产生可检测到的影响。
